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Clinical Chemistry 43: 1048-1055, 1997;
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(Clinical Chemistry. 1997;43:1048-1055.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Evaluation of two homogeneous methods for measuring high-density lipoprotein cholesterol

Yi-Chang Huang1, Jau-Tsuen Kao2 and Keh-Sung Tsai3,a

1 Department of Laboratory Medicine, Municipal Ho-Pin Hospital, 33 Sec 2, Chung-Hwa Rd., Taipei, Taiwan, R.O.C.

2 School of Medical Technology, College of Medicine, National Taiwan University, 7 Chung-Shan South Rd., 10016 Taipei, Taiwan, R.O.C.

3 Department of Laboratory Medicine, College of Medicine, National Taiwan University Hospital, National Taiwan University, 7 Chung-Shan South Rd., 10016 Taipei, Taiwan, R.O.C.
a Author for correspondence. Fax 886-2-3224263;

We evaluated the performance of two homogeneous assays for quantifying HDL cholesterol (HDL-C) and compared them with the phosphotungstic acid (PTA)/MgCl2 assay. Both homogeneous HDL-C assays were precise, having a within-run CV of <1.20% and a between-run CV of <4.07%. The HDL-C values (y) measured by the two homogeneous methods correlated well with those by the PTA/MgCl2 method (x): y = 1.00x + 64.98 mg/L, r = 0.987, Sy|x = 27.99 mg/L (n = 152) for the polyethylene glycol-modified enzymes/{alpha}-cyclodextrin sulfate (PEGME) assay (Kyowa), and y = 0.84x + 106.51 mg/L, r = 0.984, Sy|x = 26.10 mg/L (n = 152) for the polyanion–polymer/detergent (PPD) assay (Daiichi). The specificity of the PEGME method seemed better than that of the PPD method, as the PPD method was markedly interfered with by supplemental LDL-C. Addition of 20 g/L triglycerides produced a negative error of ~18% in both homogeneous assays. Bilirubin and hemoglobin had little influence on the PEGME method; hemoglobin had little effect on the PPD method. Bilirubin, however, markedly decreased the readings by the PPD method. We found the PEGME assay superior to the PPD assay for routine HDL-C testing, because the PPD assay is relatively inaccurate and not specific.


Key Words: indexing terms: methods comparison • triglycerides • atherosclerosis




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