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Clinical Chemistry 43: 930-935, 1997;
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(Clinical Chemistry. 1997;43:930-935.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Use of polymerase chain reaction to identify pneumococcal infection associated with hemorrhage and shock in two previously healthy young children

Trudy O. Messmera, Cynthia G. Whitney and Barry S. Fields

National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Atlanta, GA 30333.
a Address correspondence to this author at: Centers for Disease Control and Prevention, 1600 Clifton Rd., MS G05, Atlanta, GA 30333. Fax 404-639-3123; e-mail TYM2{at}CIDDBD2.EM.CDC.GOV

A PCR assay was developed for detection of Streptococcus pneumoniae in clinical specimens including blood and paraffinized tissues. We were able to detect one organism of purified DNA or 4.5 colony-forming units in blood. The primers did not cross-react with other upper respiratory tract streptococci or with pathogens commonly found in clinical specimens. This assay was used in an investigation of an outbreak of severe illness characterized by septic shock and hemorrhage in previously healthy children. PCR detected S. pneumoniae in cerebrospinal fluid and autopsy tissues of the two infants who died. The findings from this assay indicated that PCR offers increased specificity and sensitivity over latex agglutination and counterimmunoelectrophoresis and should prove useful in the identification of additional cases of severe illness caused by S. pneumoniae.


Key Words: indexing terms: PCR • Streptococcus pneumoniae • clinical specimens • paraffinized tissues




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Identification, prevalence and population structure of non-typable Streptococcus pneumoniae in carriage samples isolated from preschoolers attending day-care centres
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J. Moreno, E. Hernandez, O. Sanabria, and E. Castaneda
Detection and Serotyping of Streptococcus pneumoniae from Nasopharyngeal Samples by PCR-Based Multiplex Assay
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