Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 936-942, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lopez-Crapez, E.
Right arrow Articles by Grenier, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lopez-Crapez, E.
Right arrow Articles by Grenier, J.
Related Collections
Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 1997;43:936-942.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Rapid and large-scale method to detect K-ras gene mutations in tumor samples

Evelyne Lopez-Crapez1,a, Camille Chypre2, Jacqueline Saavedra2, Joseph Marchand2 and Jean Grenier1

1 C.R.L.C. Val d'Aurelle, Laboratoire de Radioanalyse, 34094 Montpellier Cédex 5, France.

2 Cis Bio International, 91192 Gif-sur-Yvette Cédex, France.
a Author for correspondence. Fax 33 4 67 63 28 73.

We have developed a rapid and large-scale method for the detection of K-ras gene mutations in tumors. First, DNA is amplified by an asymmetric PCR; second, the single-strand dinitrophenyl (DNP)-labeled amplified DNA is hybridized specifically to oligonucleotide probes affixed on a tube. Finally, perfectly matched duplexes are easily detected by a monoclonal anti-DNP antibody bearing125I. The usefulness of this technique is illustrated by analyzing K-ras codon 12 mutations in human colorectal samples. This reliable assay procedure can be applied to the rapid screening of virtually any genetic disease caused by previously described point mutations.


Key Words: indexing terms: immobilized oligonucleotide probes • point mutation • allele-specific oligonucleotide detection • polymerase chain reaction




The following articles in journals at HighWire Press have cited this article:


Home page
 Nucleic Acids ResHome page
E. Lopez-Crapez, H. Bazin, E. Andre, J. Noletti, J. Grenier, and G. Mathis
A homogeneous europium cryptate-based assay for the diagnosis of mutations by time-resolved fluorescence resonance energy transfer
Nucleic Acids Res., July 15, 2001; 29(14): e70 - e70.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
E. Lopez-Crapez, T. Livache, J. Marchand, and J. Grenier
K-ras Mutation Detection by Hybridization to a Polypyrrole DNA Chip
Clin. Chem., February 1, 2001; 47(2): 186 - 194.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.