Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 1159-1164, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tamate, K.
Right arrow Articles by Kane, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tamate, K.
Right arrow Articles by Kane, M. M.
Related Collections
Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 1997;43:1159-1164.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Direct colorimetric monoclonal antibody enzyme immunoassay for estradiol-17ß in saliva

Kenichi Tamate1,2, Margaret Charleton1, James P. Gosling3, Declan Egan4, Mutsuo Ishikawa2, Patrick F. Fottrell3 and Marian M. Kane1,a

1 National Diagnostics Centre—Bioresearch Ireland, University College, Galway, Ireland.

2 Department of Obstetrics & Gynecology, Asahikawa Medical College, Japan.

3 Department of Biochemistry, University College, Galway, Ireland.

4 Fertility Unit, Department of Obstetrics & Gynecology, University College Hospital, Galway, Ireland.
a Author for correspondence. Fax 353 91 586570; e-mail: marian. kane{at}ucg.ie

We developed a direct microtiter plate enzyme immunoassay to measure estradiol-17ß in saliva. The assay has a commercially available monoclonal antibody, raised against estradiol-17ß–6-carboxymethyloxime–bovine serum albumin, and a homologous horseradish peroxidase conjugate measured colorimetrically. The detection limit (equivalent to B0 - 3 SD) is 365 amol/well or 7.3 pmol/L when 50-µL samples are assayed. Cross-reactivity with estrone and estriol, testosterone, or progesterone is <0.2%. Estradiol-17ß was measured in daily samples over five natural menstrual cycles and eight cycles stimulated as a preliminary to in vitro fertilization, and the concentrations and fluctuations found agreed with previously published data. This method gives results in ~3 h and may be useful for fertility monitoring and management.


Key Words: indexing terms: menstrual cycle • in vitro fertilization




The following articles in journals at HighWire Press have cited this article:


Home page
 ReproductionHome page
G Wycherley, D Downey, M T Kane, and A C Hynes
A novel follicle culture system markedly increases follicle volume, cell number and oestradiol secretion
Reproduction, June 1, 2004; 127(6): 669 - 677.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.