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Clinical Chemistry 43: 1165-1171, 1997;
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(Clinical Chemistry. 1997;43:1165-1171.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Analytical and clinical evaluation of the Immulite® estradiol assay in serum from patients undergoing in vitro fertilization: estradiol increase in mature follicles

François Dancoine1,a, Gisèle Couplet1, Jacques Buvat2, Catherine Guittard3, Gérard Marcolin3 and Jean-Claude Fourlinnie1

1 Laboratoire de Biologie Médicale Drs Leduc et Ass, 17 Rue de la Digue, F-59020;
2 49 Rue de la Bassée, F 59000;
3 145 Ave. de Dunkerque, F 59000 Lille, France
a Author for correspondence. Fax +33 3 20 40 42 64.

We examined an immunoassay for estradiol (E2) on the Immulite®—an automated, random access chemiluminescent immunoassay system—to determine its accuracy and precision required for in vitro fertilization (IVF) studies. The assay, which has a reportable range from 73 to 7300 pmol/L, demonstrated good linearity under dilution, a detection limit of 44 pmol/L, and interassay CVs of 12.6% and 7.6% at 466 and 6164 pmol/L, respectively. In a retrospective analysis of 545 serum samples, the assay showed adequate agreement with an antibody-coated-tube RIA. The two E2 assays showed good agreement, even on samples from patients receiving a variety of different estrogen replacement therapies. Longitudinal studies of individual IVF cycles showed good parallelism between the automated system and the RIA, and results by the automated assay correlated well with the total number of follicles.


Key Words: indexing terms: chemiluminescence • radioimmunoassay • follicular growth • embryo transfer




The following articles in journals at HighWire Press have cited this article:


Home page
 Hum ReprodHome page
I. Tummon, J. Stemp, C. Rose, H. Vandenberghe, B. Bany, F. Tekpetey, and J. Martin
Precision and method bias of two assays for oestradiol: consequences for decisions in assisted reproduction
Hum. Reprod., May 1, 1999; 14(5): 1175 - 1177.
[Abstract] [Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.