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Clinical Chemistry 43: 1188-1195, 1997;
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(Clinical Chemistry. 1997;43:1188-1195.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Serum concentrations of free ubiquitin and multiubiquitin chains

Koji Takada1,a, Hidekazu Nasu4, Nozomu Hibi4, Yutaka Tsukada4, Toshiaki Shibasaki2, Kiyotaka Fujise3, Masahiro Fujimuro5, Hitoshi Sawada5, Hideyoshi Yokosawa5 and Kiyoshi Ohkawa1

1 Department of Biochemistry (I),
2 Department of Internal Medicine (II), and
3 Department of Internal Medicine (Kashiwa Hospital), Jikei University School of Medicine, Minato-ku, Tokyo 105, Japan.

4 Department of Research Laboratory, SRL, Inc., Komiya-cho, Hachioji-shi, Tokyo 192, Japan.

5 Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan.
a Author for correspondence. Fax 81-3-3435-1922;

Ubiquitin, which can conjugate with cellular proteins, is classified into two forms: free ubiquitin and multiubiquitin chains. The latter is active as a signal for degradation of the targeted proteins. We found both forms in human serum and, using two immunoassays, quantitated them in sera from healthy subjects and patients with some diseases. Because of putative leakage of erythrocyte ubiquitin, hemolytic serum and serum obtained after long incubation (>1–2 h) of blood at room temperature were excluded. Serum concentrations of multiubiquitin chains and free ubiquitin were substantially higher in rheumatoid arthritis and hemodialysis patients, respectively, than healthy subjects. Additionally, in acute viral hepatitis, serum multiubiquitin chain concentrations were increased in the acute phase, decreased in the recovery phase, and correlated with alanine and aspartate aminotransferase activities (r = 0.676 and 0.610, P <0.0001 and <0.001, respectively). Therefore, serum ubiquitin may have prognostic value.


Key Words: indexing terms: radioimmunoassay • enzyme-linked immunosorbent assay • hemolysis • hemodialysis • rheumatoid arthritis • acute viral hepatitis




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Copyright © 1997 by the American Association for Clinical Chemistry.