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Clinical Chemistry 43: 1321-1324, 1997;
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(Clinical Chemistry. 1997;43:1321-1324.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Apolipoprotein E genotyping by capillary electrophoretic analysis of restriction fragments

Gianluca De Bellis1,a, Giuliana Salani1, Silvia Panigone1, Ferruccio Betti1, Luigia Invernizzi1 and Massimo Luzzana2

1 Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche Avanzate, Via Fratelli Cervi 93, 20090 Segrate, Italy.

2 Università degli Studi di Milano, Dipartimento di Scienze e Tecnologie Biomediche, Via Fratelli Cervi 93, 20090 Segrate, Italy.
a Author for correspondence. Fax 39-2-26422770; e-mail debellis{at}itba.mi.cnr.it

We present the genotyping of apolipoprotein (apo) E by means of restriction fragment analysis of amplified genomic DNA by high-performance capillary electrophoresis and a replaceable non-gel-sieving matrix. This procedure streamlines the genotyping of apo E in large-scale population studies because of the automation and speed of capillary electrophoresis.


Key Words: indexing terms: genotype determination • Alzheimer disease • high-performance capillary electrophoresis




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
G. Marrazza, G. Chiti, M. Mascini, and M. Anichini
Detection of Human Apolipoprotein E Genotypes by DNA Electrochemical Biosensor Coupled with PCR
Clin. Chem., January 1, 2000; 46(1): 31 - 37.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
G. G. Donohoe, A. Salomaki, T. Lehtimaki, K. Pulkki, and V. Kairisto
Rapid Identification of Apolipoprotein E Genotypes by Multiplex Amplification Refractory Mutation System PCR and Capillary Gel Electrophoresis
Clin. Chem., January 1, 1999; 45(1): 143 - 146.
[Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.