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HyTest Ltd., Tykistokatu 6A, FIN-20520 Turku, Finland.
2
Departments of Biochemistry and Bioorganic
Chemistry, School of Biology, Moscow State University, 119899 Moscow,
Russia.
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Institute of Medical Ecology, Simpheropolskiy bull.
8, Moscow 113149, Russia.
4
Department of Biotechnology, University of Turku,
Tykistokatu 6A, FIN-20520 Turku, Finland.
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Turku University Hospital, Central Laboratory,
Kiinanmyllynkaty 4–8, FIN-20520 Turku, Finland.
a Author for correspondence. Fax +358-2-3338070; e-mail hytest{at}utu.fi
Fourteen monoclonal antibodies (mAbs) against human cardiac troponin I (cTnI) were generated by commonly used experimental techniques. All these antibodies, as well as antibody 414 (HyTest), were specific for human cTnI. Fifteen antibodies thus obtained were tested in a sandwich cTnI immunofluorescence assay (altogether 196 combinations). Ten pairs giving the highest sensitivity were selected for further investigation. The effect of TnI–TnC complex formation on antibody interaction with antigen was analyzed. The formation of TnI–TnC complex results in a significant decrease of the interaction of mAbs with TnI for seven of 10 analyzed pairs of antibodies. Using two pairs of cTnI-specific mAbs, one that recognized only free cTnI but not cTnI complexed with cTnC, and another that could be used for measurement of total cTnI (free cTnI and cTnI in complex with cTnC), we demonstrated that the main part of cTnI in serum collected from acute myocardial infarction patients is presented in the complex form. We concluded that effective and reliable immunological detection of TnI is possible only when antibodies used for assay development recognize both free TnI and TnI complexed with other troponin components.
Key Words: indexing terms: AMI diagnosis • immunoassay
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P. Datta, K. Foster, and A. Dasgupta Comparison of Immunoreactivity of Five Human Cardiac Troponin I Assays toward Free and Complexed Forms of the Antigen: Implications for Assay Discordance Clin. Chem., December 1, 1999; 45(12): 2266 - 2269. [Full Text] [PDF]
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Q. Shi, M. Ling, X. Zhang, M. Zhang, L. Kadijevic, S. Liu, and J. P. Laurino Degradation of Cardiac Troponin I in Serum Complicates Comparisons of Cardiac Troponin I Assays Clin. Chem., July 1, 1999; 45(7): 1018 - 1025. [Abstract] [Full Text] [PDF]
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D. J. Newman, Y. Olabiran, W. D. Bedzyk, S. Chance, E. G. Gorman, and C. P. Price Impact of Antibody Specificity and Calibration Material on the Measure of Agreement between Methods for Cardiac Troponin I Clin. Chem., June 1, 1999; 45(6): 822 - 828. [Abstract] [Full Text] [PDF]
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I. Giuliani, J.-P. Bertinchant, C. Granier, M. Laprade, S. Chocron, G. Toubin, J.-P. Etievent, C. Larue, and S. Trinquier Determination of Cardiac Troponin I Forms in the Blood of Patients with Acute Myocardial Infarction and Patients Receiving Crystalloid or Cold Blood Cardioplegia Clin. Chem., February 1, 1999; 45(2): 213 - 222. [Abstract] [Full Text] [PDF]
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F. S. Apple Clinical and Analytical Standardization Issues Confronting Cardiac Troponin I Clin. Chem., January 1, 1999; 45(1): 18 - 20. [Full Text] [PDF]
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A. G. Katrukha, A. V. Bereznikova, V. L. Filatov, T. V. Esakova, O. V. Kolosova, K. Pettersson, T. Lovgren, T. V. Bulargina, I. R. Trifonov, N. A. Gratsiansky, et al. Degradation of cardiac troponin I: implication for reliable immunodetection Clin. Chem., December 1, 1998; 44(12): 2433 - 2440. [Abstract] [Full Text] [PDF]
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C. Heeschen, B. U. Goldmann, Robert. H. Moeller, and C. W. Hamm Analytical performance and clinical application of a new rapid bedside assay for the detection of serum cardiac troponin I Clin. Chem., September 1, 1998; 44(9): 1925 - 1930. [Abstract] [Full Text] [PDF]
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R. H. Christenson and H. M. E. Azzazy Biochemical markers of the acute coronary syndromes Clin. Chem., August 1, 1998; 44(8): 1855 - 1864. [Abstract] [Full Text] [PDF]
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O. Hetland and K. Dickstein Cardiac troponins I and T in patients with suspected acute coronary syndrome: a comparative study in a routine setting Clin. Chem., July 1, 1998; 44(7): 1430 - 1436. [Abstract] [Full Text] [PDF]
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A. H. B. Wu, Y.-J. Feng, R. Moore, F. S. Apple, P. H. McPherson, K. F. Buechler, G. Bodor, f. t. A. A. for, and C. C. S. o. c. Standardization Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I Clin. Chem., June 1, 1998; 44(6): 1198 - 1208. [Abstract] [Full Text] [PDF]
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G. Ferrieres, C. Calzolari, J.-C. Mani, D. Laune, S. Trinquier, M. Laprade, C. Larue, B. Pau, and C. Granier Human cardiac troponin I: precise identification of antigenic epitopes and prediction of secondary structure Clin. Chem., March 1, 1998; 44(3): 487 - 493. [Abstract] [Full Text] [PDF]
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R. H. Christenson, S.-H. Duh, L. K. Newby, E. M. Ohman, R. M. Califf, C. B. Granger, S. Peck, K. S. Pieper, P. W. Armstrong, H. A. Katus, et al. Cardiac troponin T and cardiac troponin I: relative values in short-term risk stratification of patients with acute coronary syndromes Clin. Chem., March 1, 1998; 44(3): 494 - 501. [Abstract] [Full Text] [PDF]
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