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Articles |
1
INSERM U402,
2
GRENEMH,
3
Laboratoire de Biochimie A, CHU Saint-Antoine, 75571 Paris Cedex 12, France.
a Address correspondence to this author at: INSERM U402, CHU Saint-Antoine 27, rue de Chaligny, 75571 Paris Cedex 12, France. Fax +33 1.44.75.99.80.
The Hitachi L-8500A is a newly available apparatus for amino acid
(AA) analysis that allows automatic on-line mixing of
the ninhydrin reagent. The within-run precision (human plasma pools at
three different concentrations) showed CVs <3.8% except for the
lowest concentration of citrulline (4.4%), Tyr (4.5%), and
-aminobutyric acid (7.6%), and for the intermediate concentration
of Asp (8.7%). Between-run precision (CV) was <3.1% for 17 AAs and
<8.0% for 24 of 25 AAs (CV Asp = 12.0%). For retention times,
within-run precision was <0.4% and between-run precision <1.8%.
Excellent relations were found between the results from the Hitachi
L-8500A and the widely used Beckman 6300 analyzer (0.929
r 0.999). The detection was still linear at 5 µmol/L
except for Pro and hydroxyproline (20 µmol/L). The upper limit was at
least 2500 µmol/L for 13 AAs and at least 1000 µmol/L for 27 of 29
AAs (anserine = 500, Val = 600 µmol/L). Values from 100
human plasma samples agreed with previously published data. We conclude
that the results obtained with the Hitachi L-8500A are satisfactory
when compared with those of other AA analyzers utilizing the same
method. Furthermore, the Hitachi L-8500A displays several advantages
including programming flexibility, microsample capacity, low noise
plotting, ammonia filtering, and manual repacking of the analytical
column.
Key Words: indexing terms: ninhydrin reaction • cation-exchange chromatography • analytical performance evaluation
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