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Clinical Chemistry 43: 1429-1435, 1997;
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(Clinical Chemistry. 1997;43:1429-1435.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Measurement of serum iohexol by determination of iodine with inductively coupled plasma–atomic emission spectroscopy

W. Emmett Braselton1,2,a, Kirk J. Stuart2 and John M. Kruger3

1 Departments of Pharmacology and Toxicology and
2 Small Animal Clinical Sciences, and
3 Animal Health Diagnostic Laboratory, College of Veterinary Medicine, Michigan State University, E. Lansing, MI 48824.
a Address correspondence to this author at: G302 Veterinary Medical Center, Michigan State University, E. Lansing, MI 48824. Fax (517) 355-2152; e-mail braselton{at}ahdlms.cvm.msu.edu

We used inductively coupled plasma–atomic emission spectroscopy to measure serum iodine to determine plasma clearance of iohexol, an iodinated radiographic contrast agent. We determined I at 178.276 nm on the phosphorus 178.287 nm channel of the polychromator by utilization of spectrum shifter offset software, while correcting for P with the sequential P 214.914 nm emission line. Determination of I on the polychromator provided excellent precision in the measurement of serum I, even though the interelement correction of P was done with a sequential P line. Total imprecision (CV) (n = 13) was 16% (at 13.7 mg/L I), 8.6% (28.7 mg/L), 3.6% (59.0 mg/L), 2.6% (120.5 mg/L), 1.7% (237.8 mg/L), 1.2% (478.7 mg/L), and 1.8% (597 mg/L). The linear range was 15 to 600 mg/L. Iohexol added to serum (mg/L I) and recoveries (%) were 15 (91.3%), 30 (95.7%), 60 (98.3%), 120 (100.4%), 240 (99.1%), 480 (99.7%), and 600 (99.5%). Studies on dogs and cats administered a single intravenous injection of iohexol indicated that a dose of 300 mg I/kg body weight was sufficient for measurement of glomerular filtration rate by using a single compartment model for plasma clearance with three samples drawn 3 to 7 h after treatment. With this protocol, correlation coefficients were >0.99 on the ß phase of the plasma disappearance curve.


Key Words: indexing terms: glomerular filtration rate • iodine compounds • contrast media




The following articles in journals at HighWire Press have cited this article:


Home page
 J Am Coll CardiolHome page
I. Michishita and Z. Fujii
A Novel Contrast Removal System From the Coronary Sinus Using an Adsorbing Column During Coronary Angiography in a Porcine Model
J. Am. Coll. Cardiol., May 2, 2006; 47(9): 1866 - 1870.
[Abstract] [Full Text] [PDF]


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