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Clinical Chemistry 43: 1442-1447, 1997;
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(Clinical Chemistry. 1997;43:1442-1447.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Binding of nitric oxide to thiols and hemes in hemoglobin H: implications for {alpha}-thalassemia and hypertension

Poluru L. Reddy1, Lemuel J. Bowie1,a and Steven Callistein2

1 Clinical Laboratories, Evanston Hospital, 2650 Ridge Ave., Evanston, IL 60201-1783.

2 1323 W. Henderson, Chicago, IL 60657.
a Author for correspondence. Fax 847-570-2969; e-mail lem{at}merle.acns.nwu.edu

Our earlier studies suggested an association between {alpha}-thalassemia and hypertension. We postulated that this association might involve trapping of the vasodilator nitric oxide (NO) by hemoglobin (Hb). Hb A has recently been shown to carry NO on its sulfhydryl groups in addition to its hemes. In this report we studied the interaction of purified Hb H as well as Hb A with NO. The number of reactive sulfhydryls were determined spectrophotometrically with bis-dithionitrobenzoate. Spectral studies and nitrosothiol measurements after treatment with NO or nitrosothiols indicated that all eight reactive sulfhydryls of Hb H were capable of binding NO. Hb A, however, was only able to bind and transfer two molecules of NO per tetramer. These findings support the biochemical basis for the association between {alpha}-thalassemia and hypertension.


Key Words: indexing terms: hemoglobin • thalassemia • sulfhydryl compounds • nitroso compounds • methemoglobin




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Biol. Chem.Home page
T. J. McMahon, A. Exton Stone, J. Bonaventura, D. J. Singel, and J. Solomon Stamler
Functional Coupling of Oxygen Binding and Vasoactivity in S-Nitrosohemoglobin
J. Biol. Chem., May 26, 2000; 275(22): 16738 - 16745.
[Abstract] [Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.