Clinical Chemistry
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Clinical Chemistry 43: 1448-1454, 1997;
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(Clinical Chemistry. 1997;43:1448-1454.)
© 1997 American Association for Clinical Chemistry, Inc.

Case Reports

Prostate-specific antigen expression in a case of intracystic carcinoma of the breast: characterization of immunoreactive protein and literature surveys

Ferdinando Mannello1,a, Maurizio Sebastiani2, Silvana Amati3 and Giancarlo Gazzanelli1

1 Istituto di Istologia ed Analisi di Laboratorio, Facoltà di Scienze M.F.N., Università Studi, Via Zeppi, 61029 Urbino (PS), Italy.

2 Centro di Senologia, AUSL 1, Pesaro, Italy.

3 Istituto di Istologia ed Anatomia, Facoltà di Medicina, Università, Ancona, Italy.
a Author for correspondence. Fax +39-722-322370;

A case is presented of female breast intracystic carcinoma with prostate-specific antigen (PSA) expressed in high amounts in aspirated cystic fluid (55 µg/L). Tumor extract analysis revealed the presence of both estrogen and progesterone receptors (0.38 and 1.87 nmol/L, respectively) and high quantities of PSA too (19.52 µg/L). Chromatographic analysis of cystic fluid revealed two peaks of PSA, at the expected positions for free and bound serine protease. A major proportion of 33-kDa free form was also confirmed by Western blotting analysis. Free PSA was heat-stable at 56 °C and displayed no change after freezing–thawing. These findings are discussed in the context of a detailed literature survey. Our data support the contention that PSA immunoreactivity in intracystic fluid of breast carcinoma is partly the result of secretory activity by the neoplastic cells and that the steroid receptors can also modulate its expression.


Key Words: indexing terms: {alpha}1-antichymotrypsin • breast cyst fluid • fibrocystic disease • serine proteases • estrogen receptor • progesterone receptor




The following articles in journals at HighWire Press have cited this article:


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 J. Clin. Endocrinol. Metab.Home page
M. Malatesta, F. Mannello, F. Luchetti, F. Marcheggiani, L. Condemi, S. Papa, and G. Gazzanelli
Prostate-Specific Antigen Synthesis and Secretion by Human Placenta: A Physiological Kallikrein Source during Pregnancy
J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 317 - 321.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
F. Mannello, M. Malatesta, M. Sebastiani, S. Battistelli, and G. Gazzanelli
Molecular Forms and Ultrastructural Localization of Prostate-specific Antigen in Nipple Aspirate Fluids,
Clin. Chem., December 1, 1999; 45(12): 2263 - 2266.
[Full Text] [PDF]

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 Clin. Chem.Home page
F. Mannello, M. Malatesta, F. Luchetti, S. Papa, S. Battistelli, and G. Gazzanelli
Immunoreactivity, Ultrastructural Localization, and Transcript Expression of Prostate-specific Antigen in Human Neuroblastoma Cell Lines
Clin. Chem., January 1, 1999; 45(1): 78 - 84.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Mannello, F. Luchetti, D. Lancioli, S. Battistelli, S. Papa, and G. Gazzanelli
Prostate-specific Antigen Expression in Neoplastic Human Myeloid Cell Lines
Clin. Chem., September 1, 1998; 44(9): 1991 - 1993.
[Full Text] [PDF]

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 Clin. Chem.Home page
F. Mannello, M. Malatesta, E. Fusco, G. Bianchi, A. Cardinali, and G. Gazzanelli
Biochemical Characterization and Immunolocalization of Prostate-specific Antigen in Human Term Placenta
Clin. Chem., August 1, 1998; 44(8): 1735 - 1737.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Mannello, F. Luchetti, E. Falcieri, S. Papa, and G. Gazzanelli
Prostate-specific Antigen Expression in Neuroblastoma Cell Lines
Clin. Chem., June 1, 1998; 44(6): 1362 - 1363.
[Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.