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Clinical Chemistry 43: 1738-1743, 1997;
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(Clinical Chemistry. 1997;43:1738-1743.)
© 1997 American Association for Clinical Chemistry, Inc.


Oak Ridge Conference

Interferences of o-raffinose cross-linked hemoglobin in three methods for serum creatinine

Arlene C. Y. Alia, Chrystina C. Mihas and Janet A. Campbell

a Author for correspondence: fax 416-798-0152.


Abstract

The interferences of o-raffinose cross-linked hemoglobin (HemolinkTM) were examined and compared in two serum creatinine methods on the Hitachi 717 [Boehringer Mannheim (BMC) and Synermed] and in an enzymatic creatinine method on the Vitros 750 (Johnson & Johnson). Interference was considered significant when the change in creatinine concentration from the control exceeded the 95% confidence limits of each method. Significant interference was observed for the BMC/Hitachi 717 method with Hemolink >=5 g/L. No interference was observed for the Synermed/Hitachi 717 assay with Hemolink up to 30 g/L in normal samples and up to 50 g/L in samples with increased creatinine. No interference was observed for the Vitros 750 assay with Hemolink up to 50 g/L at both normal and increased concentrations of creatinine. Although the BMC/Hitachi 717 method was considered unacceptable, the Synermed/Hitachi 717 and the Vitros 750 methods allow accurate quantification of serum creatinine in the presence of Hemolink.




The following articles in journals at HighWire Press have cited this article:


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A. A. Ali, G. S. Ali, J. M. Steinke, and A. P. Shepherd
Co-Oximetry Interference by Hemoglobin-Based Blood Substitutes
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C. M. Balion, P. A. Champagne, and A. C. Y. Ali
Evaluation of HemogloBindTM for Removal of o-Raffinose Cross-Linked Hemoglobin (HemolinkTM) from Serum
Clin. Chem., September 1, 1997; 43(9): 1796 - 1798.
[Full Text]




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