Clinical Chemistry
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Clinical Chemistry 43: 1764-1770, 1997;
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(Clinical Chemistry. 1997;43:1764-1770.)
© 1997 American Association for Clinical Chemistry, Inc.

Oak Ridge Conference

Detection of hepatitis B surface antigen in whole blood by coupled particle light scattering (CopalisTM)

Michael J. Beneckya, Diane R. Post, Susan M. Schmitt and Manish S. Kochar

Sienna Biotech, Inc., 9115 Guilford Rd., Suite 180, Columbia, MD 21046.
a Author for correspondence. Fax 301-497-8796;


Abstract

Coupled particlelight scattering (CopalisTM) is a homogeneous immunoassay technology that permits simultaneous determination of multiple analytes in serum, plasma, or whole blood. Copalis differentiates monomeric latex microparticles from latex aggregates and cells on the basis of their unique light scatter properties. Copalis readily discriminates small (~0.1 µm) differences in latex microparticle size. Therefore, multiple simultaneous assays are configured by the use of mixtures of different-size latex microparticles. The Copalis research immunoassay for hepatitis B surface antigen (HBsAg) is configured in a sandwich format where the extent of light scatter histogram broadening due to HBsAg-mediated binding of colloidal gold to latex provides the basis for antigen quantification. Simultaneous Copalis forward- and wide-angle light scatter measurements allow discrimination of latex microparticles from the cell components of whole blood. Consequently, direct detection of HBsAg in unprocessed whole-blood samples by Copalis is feasible.




The following articles in journals at HighWire Press have cited this article:


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 Proc. Natl. Acad. Sci. USAHome page
J. Baudry, C. Rouzeau, C. Goubault, C. Robic, L. Cohen-Tannoudji, A. Koenig, E. Bertrand, and J. Bibette
Acceleration of the recognition rate between grafted ligands and receptors with magnetic forces
PNAS, October 31, 2006; 103(44): 16076 - 16078.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
M. J. Benecky, K. L. McKinney, K. M. Peterson, and J. Q. Kamerud
Simultaneous Detection of Multiple Analytes Using Copalis Technology: A Reduction to Practice
Clin. Chem., September 1, 1998; 44(9): 2052 - 2054.
[Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.