Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 1764-1770, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Benecky, M. J.
Right arrow Articles by Kochar, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Benecky, M. J.
Right arrow Articles by Kochar, M. S.
Related Collections
Right arrow Oak Ridge Conference
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 1997;43:1764-1770.)
© 1997 American Association for Clinical Chemistry, Inc.

Oak Ridge Conference

Detection of hepatitis B surface antigen in whole blood by coupled particle light scattering (CopalisTM)

Michael J. Beneckya, Diane R. Post, Susan M. Schmitt and Manish S. Kochar

Sienna Biotech, Inc., 9115 Guilford Rd., Suite 180, Columbia, MD 21046.
a Author for correspondence. Fax 301-497-8796;


Abstract

Coupled particlelight scattering (CopalisTM) is a homogeneous immunoassay technology that permits simultaneous determination of multiple analytes in serum, plasma, or whole blood. Copalis differentiates monomeric latex microparticles from latex aggregates and cells on the basis of their unique light scatter properties. Copalis readily discriminates small (~0.1 µm) differences in latex microparticle size. Therefore, multiple simultaneous assays are configured by the use of mixtures of different-size latex microparticles. The Copalis research immunoassay for hepatitis B surface antigen (HBsAg) is configured in a sandwich format where the extent of light scatter histogram broadening due to HBsAg-mediated binding of colloidal gold to latex provides the basis for antigen quantification. Simultaneous Copalis forward- and wide-angle light scatter measurements allow discrimination of latex microparticles from the cell components of whole blood. Consequently, direct detection of HBsAg in unprocessed whole-blood samples by Copalis is feasible.




The following articles in journals at HighWire Press have cited this article:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Baudry, C. Rouzeau, C. Goubault, C. Robic, L. Cohen-Tannoudji, A. Koenig, E. Bertrand, and J. Bibette
Acceleration of the recognition rate between grafted ligands and receptors with magnetic forces
PNAS, October 31, 2006; 103(44): 16076 - 16078.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. J. Benecky, K. L. McKinney, K. M. Peterson, and J. Q. Kamerud
Simultaneous Detection of Multiple Analytes Using Copalis Technology: A Reduction to Practice
Clin. Chem., September 1, 1998; 44(9): 2052 - 2054.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.