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Enzymes and Protein Markers |
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Abteilung für Klinische Chemie und Zentrallaboratorium, Baldingerstr., and
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Medizinisches Zentrum für Nervenheilkunde, Funktionsbereich Neurochemie, Rudolf-Bultmannstr. 8, Philipps-Universität, D-35033 Marburg, Germany.
Serum concentration of carbohydrate-deficient transferrin (CDT) is used for laboratory diagnosis of chronic alcohol abuse. Using isoelectric focusing for validation of the initial isotransferrin fractionation step involved in the determination of CDT by the CDTect assay, we found a complete in vitro iron saturation of transferrin and sufficient stability of the transferrin iron load during column passage; effective separation of non-CDT-isotransferrins and CDT-isotransferrins at the microcolumns; partial coelution of trisialo-Fe2-transferrin, which did not significantly affect CDT measurement; partial retention of CDT-isotransferrins, especially disialo-Fe2-transferrin, which may cause falsely negative results for CDT at the upper reference limits; good precision of the isotransferrin fractionation step; and no significant effects of low concentrations of serum protein and transferrin. We strongly urge standardization of CDT analysis and suggest isoelectric focusing for validation of CDT analysis methods and verification of odd results.
The following articles in journals at HighWire Press have cited this article:
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  J. P. Bergstrom and A. Helander Clinical Characteristics of Carbohydrate-Deficient Transferrin (%Disialotransferrin) Measured by HPLC: Sensitivity, Specificity, Gender Effects, and Relationship with other Alcohol Biomarkers Alcohol Alcohol., July 1, 2008; 43(4): 436 - 441. [Abstract] [Full Text] [PDF]
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P. Anttila, K. Jarvi, J. Latvala, J. E. Blake, and O. Niemela DIAGNOSTIC CHARACTERISTICS OF DIFFERENT CARBOHYDRATE-DEFICIENT TRANSFERRIN METHODS IN THE DETECTION OF PROBLEM DRINKING: EFFECTS OF LIVER DISEASE AND ALCOHOL CONSUMPTION Alcohol Alcohol., September 1, 2003; 38(5): 415 - 420. [Abstract] [Full Text] [PDF]
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 T. Arndt Asialotransferrin--An Alternative to Carbohydrate-deficient Transferrin? Clin. Chem., June 1, 2003; 49(6): 1022 - 1023. [Full Text] [PDF]
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F. J. Legros, V. Nuyens, M. Baudoux, K. Zouaoui Boudjeltia, J.-L. Ruelle, J. Colicis, F. Cantraine, and J.-P. Henry Use of Capillary Zone Electrophoresis for Differentiating Excessive from Moderate Alcohol Consumption Clin. Chem., March 1, 2003; 49(3): 440 - 449. [Abstract] [Full Text] [PDF]
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T. Arndt and J. Kropf Alcohol Abuse and Carbohydrate-deficient Transferrin Analysis: Are Screening and Confirmatory Analysis Required? Clin. Chem., November 1, 2002; 48(11): 2072 - 2074. [Full Text] [PDF]
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U. Turpeinen, T. Methuen, H. Alfthan, K. Laitinen, M. Salaspuro, and U.-H. Stenman Comparison of HPLC and Small Column (CDTect) Methods for Disialotransferrin Clin. Chem., October 1, 2001; 47(10): 1782 - 1787. [Abstract] [Full Text] [PDF]
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A. Helander, G. Eriksson, H. Stibler, and J.-O. Jeppsson Interference of Transferrin Isoform Types with Carbohydrate-deficient Transferrin Quantification in the Identification of Alcohol Abuse Clin. Chem., July 1, 2001; 47(7): 1225 - 1233. [Abstract] [Full Text] [PDF]
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B. Wuyts, J. R. Delanghe, I. Kasvosve, A. Wauters, H. Neels, and J. Janssens Determination of Carbohydrate-deficient Transferrin Using Capillary Zone Electrophoresis Clin. Chem., February 1, 2001; 47(2): 247 - 255. [Abstract] [Full Text] [PDF]
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T. Arndt Carbohydrate-deficient Transferrin as a Marker of Chronic Alcohol Abuse: A Critical Review of Preanalysis, Analysis, and Interpretation Clin. Chem., January 1, 2001; 47(1): 13 - 27. [Abstract] [Full Text] [PDF]
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K. Scouller, K. M. Conigrave, P. Macaskill, L. Irwig, and J. B. Whitfield Should We Use Carbohydrate-deficient Transferrin instead of {gamma}-Glutamyltransferase for Detecting Problem Drinkers? A Systematic Review and Metaanalysis Clin. Chem., December 1, 2000; 46(12): 1894 - 1902. [Abstract] [Full Text] [PDF]
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R. Hackler, T. Arndt, A. Helwig-Rolig, J. Kropf, A. Steinmetz, and J. R. Schaefer Investigation by Isoelectric Focusing of the Initial Carbohydrate-deficient Transferrin (CDT) and non-CDT Transferrin Isoform Fractionation Step Involved in Determination of CDT by the ChronAlcoI.D. Assay Clin. Chem., April 1, 2000; 46(4): 483 - 492. [Abstract] [Full Text] [PDF]
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H. Henry, F. Froehlich, R. Perret, J.-D. Tissot, B. Eilers-Messerli, D. Lavanchy, C. Dionisi-Vici, J.-J. Gonvers, and C. Bachmann Microheterogeneity of Serum Glycoproteins in Patients with Chronic Alcohol Abuse Compared with Carbohydrate-deficient Glycoprotein Syndrome Type I Clin. Chem., September 1, 1999; 45(9): 1408 - 1413. [Abstract] [Full Text] [PDF]
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A. Helander Absolute or Relative Measurement of Carbohydrate-deficient Transferrin in Serum? Experiences with Three Immunological Assays Clin. Chem., January 1, 1999; 45(1): 131 - 135. [Full Text] [PDF]
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J. B. Whitfield, L. M. Fletcher, T. L. Murphy, L. W. Powell, J. Halliday, A. C. Heath, and N. G. Martin Smoking, obesity, and hypertension alter the dose–response curve and test sensitivity of carbohydrate-deficient transferrin as a marker of alcohol intake Clin. Chem., December 1, 1998; 44(12): 2480 - 2489. [Abstract] [Full Text] [PDF]
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A. Helander, E. Vabo, K. Levin, and S. Borg Intra- and interindividual variability of carbohydrate-deficient transferrin, {gamma}-glutamyltransferase, and mean corpuscular volume in teetotalers Clin. Chem., October 1, 1998; 44(10): 2120 - 2125. [Abstract] [Full Text] [PDF]
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T. Arndt, R. Hackler, and P. Bean Evaluation of Carbohydrate-deficient Transferrin Dr. Bean responds: Clin. Chem., May 1, 1998; 44(5): 1069 - 1071. [Full Text] [PDF]
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