Clinical Chemistry
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Clinical Chemistry 44: 78-85, 1998;
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Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 1998;44:78-85.)
© 1998 American Association for Clinical Chemistry, Inc.

Endocrinology and Metabolism

Immunoradiometric assay of succinylated corticotropin: an improved method for quantification of ACTH

Gilles Kertesza, Béatrice Bourcier, Hélène Cailla, and Frédéric Jean

a Author for correspondence. Fax 334 91 17 27 40.

In this paper we describe the development and the evaluation of a new type of immunoassay for human corticotropin (ACTH). We succeeded, by using an original approach based upon immunization with ACTH derivatized with succinic anhydride, in raising monoclonal antibodies against this poorly immunogenic peptide. Three of the antibodies were selected to develop an immunoassay for ACTH. The assay requires the prior succinylation of the plasma samples for optimal sensitivity and specificity. This acylation treatment is fast, reproducible, and, in addition, improves the stability of the ACTH molecule in plasma, thus facilitating sample handling. The assay is performed in only 3 h with a detection limit of 0.7 ng/L. Analytical evaluation showed excellent specificity, reproducibility, and reliability. A comparison with two commonly used but time-consuming ACTH IRMAs was carried out by assaying several plasma samples in parallel and gave in both cases very good correlation.




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M. Boscaro, L. Barzon, and N. Sonino
The Diagnosis of Cushing's Syndrome: Atypical Presentations and Laboratory Shortcomings
Arch Intern Med, November 13, 2000; 160(20): 3045 - 3053.
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