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Hematology |
Department of Pathology, Virginia Commonwealth University/Medical College of Virginia, Richmond, VA.
a Address correspondence to this author at: Department of Pathology, Medical College of Virginia, P.O. Box 980250, Richmond, VA 23298-0250. Fax 804-828-2812; e-mail JBENEZRA{at}HSC.VCU.EDU.
Urinalysis is a high-volume procedure that currently requires significant labor to examine microscopic sediment. We evaluated the Sysmex UF-100 automated urinalysis analyzer for performing this task. Instrument accuracy was assessed by comparing continuous counts of microscopic elements from the UF-100 with ranges of cells (per low-power field or high-power field) from manual microscopy performed on centrifuged urines. Counts showed good agreement between methods (gamma statistic: 0.880–0.970) for all microscopic elements in 252 urine samples. Within-run imprecision of cell counts expressed as CV (mean cell count/µL) was for erythrocytes (RBC) 31% (5), 18% (50), 2.4% (800); for leukocytes (WBC) 14% (10), 11% (100), 8.5% (400); for squamous epithelial cells (SEC) 18% (5), 12% (30), 7.0% (100); for casts 45% (1), 17% (4); for bacteria 2–12% (entire range of 40–2500). Between-run imprecision on quality-control cell suspensions expressed as CV (mean cell count/µL) was for RBC 6.1% (50), 2.7% (256); for WBC 26.9% (54), 4.9% (228). Cells counted on dilution were 99.1% of expected for RBC, 102.0% for WBC, and 121.8% for bacteria. Carryover was <0.04% for RBC, <0.03% for WBC, <0.14% for SEC, <0.29% for bacteria. We conclude that the UF-100 can automatically perform reliable quantitative microscopic urinalysis in batches without operator interaction.
The following articles in journals at HighWire Press have cited this article:
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M. Gai, G. B. Piccoli, G. P. Segoloni, and G. Lanfranco Microscopic Urinalysis and Automated Flow Cytometry in a Nephrology Laboratory Clin. Chem., September 1, 2003; 49(9): 1559 - 1560. [Full Text] [PDF]
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C. Ottiger and A. R. Huber Quantitative Urine Particle Analysis: Integrative Approach for the Optimal Combination of Automation with UF-100 and Microscopic Review with KOVA Cell Chamber Clin. Chem., April 1, 2003; 49(4): 617 - 623. [Abstract] [Full Text] [PDF]
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J. Penders, T. Fiers, and J. R. Delanghe Quantitative Evaluation of Urinalysis Test Strips Clin. Chem., December 1, 2002; 48(12): 2236 - 2241. [Abstract] [Full Text] [PDF]
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R. Ziebig, A. Lun, and P. Sinha Leukocyte Counts in Cerebrospinal Fluid with the Automated Hematology Analyzer CellDyn 3500 and the Urine Flow Cytometer UF-100 Clin. Chem., February 1, 2000; 46(2): 242 - 247. [Abstract] [Full Text] [PDF]
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A. Lun, R. Ziebig, H. Hammer, U. Otting, G. Filler, and P. Sinha Reference Values for Neonates and Children for the UF-100 Urine Flow Cytometer Clin. Chem., October 1, 1999; 45(10): 1879 - 1880. [Full Text] [PDF]
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A. Lun, R. Ziebig, F. Priem, G. Filler, and P. Sinha Routine Workflow for Use of Urine Strips and Urine Flow Cytometer UF-100 in the Hospital Laboratory,1 Clin. Chem., August 1, 1999; 45(8): 1305 - 1307. [Full Text] [PDF]
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M. R. Langlois, J. R. Delanghe, S. R. Steyaert, K. C. Everaert, and M. L. De Buyzere Automated Flow Cytometry Compared with an Automated Dipstick Reader for Urinalysis Clin. Chem., January 1, 1999; 45(1): 118 - 122. [Abstract] [Full Text] [PDF]
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