Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 44: 2148-2151, 1998;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scheffer, P. G.
Right arrow Articles by Teerlink, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scheffer, P. G.
Right arrow Articles by Teerlink, T.
Related Collections
Right arrow Lipids, Lipoproteins, and Cardiovascular Risk Factors
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 1998;44:2148-2151.)
© 1998 American Association for Clinical Chemistry, Inc.

Lipids and Lipoproteins

Measurement of LDL particle size in whole plasma and serum by high performance gel-filtration chromatography using a fluorescent lipid probe

Peter G. Scheffer1,a, Stephan J. L. Bakker2, Robert J. Heine2, and Tom Teerlink1

Departments of
1 Clinical Chemistry and
2 Endocrinology, Research Institute for Endocrinology, Reproduction and Metabolism, Academic Hospital Vrije Universiteit, P. O. Box 7057, 1007 MB Amsterdam, The Netherlands.
a Author for correspondence. Fax 31-20-4443895;

We have recently described a technique for measuring LDL size by high performance gel-filtration chromatography (HPGC) with UV detection (Scheffer et al., Clin Chem 1997;43:1904–12). A drawback of this method is the necessity of LDL isolation before chromatography. We now describe a modification of this method based on selective detection of lipoproteins by postcolumn labeling with parinaric acid, a fluorescent lipid probe. Measuring the size of isolated LDL by HPGC in 56 subjects, we obtained diameters of 25.72 ± 0.60 nm with UV detection and of 25.74 ± 0.58 nm with fluorescence detection. The modified method is suitable for LDL size measurement in whole plasma or serum. LDL sizes measured in whole plasma correlated strongly with the respective values in isolated LDL (r = 0.976) but were on average 0.18 nm larger (P < 0.001). CVs for within- and between-series imprecision were <0.25%. The present method requires only 5 µL plasma or serum without sample preparation and is suitable for the unattended analysis of large series of samples.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
D. C. Rambaldi, A. Zattoni, S. Casolari, P. Reschiglian, D. Roessner, and C. Johann
An analytical method for size and shape characterization of blood lipoproteins.
Clin. Chem., November 1, 2007; 53(11): 2026 - 2029.
[Full Text] [PDF]

Home page
 CirculationHome page
R. Carmena, P. Duriez, and J.-C. Fruchart
Atherogenic Lipoprotein Particles in Atherosclerosis
Circulation, June 15, 2004; 109(23_suppl_1): III-2 - III-7.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
T. Teerlink, P. G. Scheffer, S. J. L. Bakker, and R. J. Heine
Combined data from LDL composition and size measurement are compatible with a discoid particle shape
J. Lipid Res., May 1, 2004; 45(5): 954 - 966.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Association for Clinical Chemistry.