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Clinical Chemistry 44: 2148-2151, 1998;
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(Clinical Chemistry. 1998;44:2148-2151.)
© 1998 American Association for Clinical Chemistry, Inc.

Lipids and Lipoproteins

Measurement of LDL particle size in whole plasma and serum by high performance gel-filtration chromatography using a fluorescent lipid probe

Peter G. Scheffer1,a, Stephan J. L. Bakker2, Robert J. Heine2, and Tom Teerlink1

Departments of
1 Clinical Chemistry and
2 Endocrinology, Research Institute for Endocrinology, Reproduction and Metabolism, Academic Hospital Vrije Universiteit, P. O. Box 7057, 1007 MB Amsterdam, The Netherlands.
a Author for correspondence. Fax 31-20-4443895;

We have recently described a technique for measuring LDL size by high performance gel-filtration chromatography (HPGC) with UV detection (Scheffer et al., Clin Chem 1997;43:1904–12). A drawback of this method is the necessity of LDL isolation before chromatography. We now describe a modification of this method based on selective detection of lipoproteins by postcolumn labeling with parinaric acid, a fluorescent lipid probe. Measuring the size of isolated LDL by HPGC in 56 subjects, we obtained diameters of 25.72 ± 0.60 nm with UV detection and of 25.74 ± 0.58 nm with fluorescence detection. The modified method is suitable for LDL size measurement in whole plasma or serum. LDL sizes measured in whole plasma correlated strongly with the respective values in isolated LDL (r = 0.976) but were on average 0.18 nm larger (P < 0.001). CVs for within- and between-series imprecision were <0.25%. The present method requires only 5 µL plasma or serum without sample preparation and is suitable for the unattended analysis of large series of samples.




The following articles in journals at HighWire Press have cited this article:


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R. Carmena, P. Duriez, and J.-C. Fruchart
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T. Teerlink, P. G. Scheffer, S. J. L. Bakker, and R. J. Heine
Combined data from LDL composition and size measurement are compatible with a discoid particle shape
J. Lipid Res., May 1, 2004; 45(5): 954 - 966.
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