Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 44: 2183-2190, 1998;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shepherd, A. P.
Right arrow Articles by Steinke, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shepherd, A. P.
Right arrow Articles by Steinke, J. M.
Related Collections
Right arrow Laboratory Management
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 1998;44:2183-2190.)
© 1998 American Association for Clinical Chemistry, Inc.

Automation and Analytical Techniques

CO-Oximetry interference by perflubron emulsion: comparison of hemolyzing and nonhemolyzing instruments

A. P. Shepherd1,2,a, and J. M. Steinke1,2

1 Department of Physiology, University of Texas Health Science Center, San Antonio, Texas 78284-7756.

2 Avox Systems, Inc., San Antonio, Texas 78015.
a Author for correspondence. Fax 210-567–4410; e-mail shepherd{at}uthscsa.edu.

Perflubron emulsion is expected to be in clinical use soon as a non-hemoglobin blood substitute. A preliminary report indicates that this new oxygen-carrying fluorocarbon interferes with the measurements of CO-oximeters. Therefore, we have quantified the interference that perflubron causes in the measurements of eight widely used oximeters and CO-oximeters. The AVL Omni 6, CC270, IL482, IL682, and OSM3 are conventional CO-oximeters that hemolyze blood samples before analyzing them. In contrast, the AVOXimeters 1000 and 4000 and the IL Synthesis 35 make their measurements without hemolyzing the samples. Because perflubron is expected to be used most frequently on surgical patients in a hemodiluted state, we conducted all tests on human erythrocytes suspended in plasma at a hemoglobin concentration standardized to 70 g/L (7 g/dL) and with oxyhemoglobin saturation set at 97%. When perflubron was added to the blood samples, the nonhemolyzing CO-oximeters were not seriously affected by perflubron concentrations in and above the therapeutic range. In contrast, some of the hemolyzing CO-oximeters experienced concentration-dependent interference in their measurements of all analytes except total hemoglobin concentration. Thus, we conclude that the nonhemolyzing CO-oximeters provide an effective means for determining whether a hemolyzing CO-oximeter is experiencing clinically important interference in blood from patients receiving perflubron.




The following articles in journals at HighWire Press have cited this article:


Home page
 Anesth. Analg.Home page
A. A. Ali, G. S. Ali, J. M. Steinke, and A. P. Shepherd
Co-Oximetry Interference by Hemoglobin-Based Blood Substitutes
Anesth. Analg., April 1, 2001; 92(4): 863 - 869.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. G. Toffaletti and R. F. Wildermann
Use of Turbidity-Correction Algorithm Eliminates the Effect of Perflubron Emulsion on CO-Oximeter Results
Clin. Chem., January 1, 2000; 46(1): 136 - 137.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Association for Clinical Chemistry.