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Clinical Chemistry 44: 2191-2194, 1998;
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(Clinical Chemistry. 1998;44:2191-2194.)
© 1998 American Association for Clinical Chemistry, Inc.

Automation and Analytical Techniques

Rapid pathogen detection using a microchip PCR array instrument

Phillip Belgrader1,a, William Benett1, Dean Hadley1, Gary Long2, Raymond Mariella, Jr.1, Fred Milanovich1, Shanavaz Nasarabadi1, William Nelson2, James Richards1, and Paul Stratton1

1 Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 7000 East Ave., Livermore, CA 94551.

2 Naval Medical Research Institute, 8901 Wisconsin Ave., Bethesda, MD 20814.
a Address correspondence to this author at: LLNL, BBRP, P.O. Box 808, L-452, Livermore, CA 94551. Fax 925-422-2282; e-mail belgrader1{at}llnl.gov.

An array of PCR microchips for rapid, parallel testing of samples for pathogenic microbes is described. The instrument, called the Advanced Nucleic Acid Analyzer (ANAA), utilizes 10 silicon reaction chambers with thin-film resistive heaters and solid-state optics. Features of the system include efficient heating and real-time monitoring, low power requirements for battery operation, and no moving parts for reliability and ruggedness. We analyzed cultures of Erwinia herbicola vegetative cells, Bacillus subtilis spores, and MS2 virions, which simulated pathogenic microbes such as Yersinia pestis, Bacillus anthracis spores, and Venezuelan equine encephalitis, respectively. Detection of microbes was achieved in as little as 16 min with detection limits of 105–107 organisms/L (102–104 organisms/mL).




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Copyright © 1998 by the American Association for Clinical Chemistry.