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Clinical Chemistry 44: 2243-2248, 1998;
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(Clinical Chemistry. 1998;44:2243-2248.)
© 1998 American Association for Clinical Chemistry, Inc.

Molecular Diagnostics and Genetics

K-ras gene mutations in the diagnosis of fine-needle aspirates of pancreatic masses: prospective study using two techniques with different detection limits

Josefina Mora1,a, Pere Puig1, Jaume Boadas2, Eulàlia Urgell1, Enric Montserrat3, Enrique Lerma4, Francesc González-Sastre1, Fèlix Lluís5,6, Antoni Farré2,6, and Gabriel Capellá6

Departments of
1 Clinical Biochemistry,
2 Gastroenterology,
3 Radiology,
4 Pathology, and
5 Surgery, and
6 Gastrointestinal Research Laboratory, Hospital de la Santa Creu i Sant Pau, Antoni M. Claret, 167, 08025 Barcelona, Spain.
a Author for correspondence. Fax 34-3-2919196; e-mail lig{at}santpau.es.

Detection of K-ras mutations may be useful in the evaluation of pancreatic cancer. The aim of this study was to assess, in a prospective design, the diagnostic utility of K-ras mutation analysis in 62 consecutive fine-needle aspirates of pancreatic masses, using two PCR-based techniques—standard and enriched—with detection limits of a mutant allele in the presence of 102 or 103 wild-type alleles, respectively. Cytology alone offered a diagnostic sensitivity of 75%. The enriched higher sensitivity detection technique, in combination with cytology, offered a diagnostic sensitivity of 91% without false positives. The molecular analysis would have contributed to diagnosis in an additional 14 cases of pancreatic cancer. The standard technique contributed to diagnosis in an additional 9 cases. These results strongly support the use of the enriched method of detecting K-ras mutations as a complement to cytology in the evaluation of pancreatic masses.




The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
J. Mora, E. Urgell, A. Farre, L. Comas, E. Montserrat, and F. Gonzalez-Sastre
Agreement between K-ras Sequence Variations Detected in Plasma and Tissue DNA in Pancreatic and Colorectal Cancer.
Clin. Chem., July 1, 2006; 52(7): 1448 - 1449.
[Full Text] [PDF]

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 Cold Spring Harb Symp Quant BiolHome page
D.A. TUVESON and S.R. HINGORANI
Ductal Pancreatic Cancer in Humans and Mice
Cold Spring Harb Symp Quant Biol, January 1, 2005; 70(0): 65 - 72.
[Abstract] [PDF]

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 JCOHome page
M. Esteller, S. Gonzalez, R. A. Risques, E. Marcuello, R. Mangues, J. R. Germa, J. G. Herman, G. Capella, and M. A. Peinado
K-ras and p16 Aberrations Confer Poor Prognosis in Human Colorectal Cancer
J. Clin. Oncol., January 15, 2001; 19(2): 299 - 304.
[Abstract] [Full Text] [PDF]


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