Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 44: 2249-2255, 1998;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (35)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ogura, M.
Right arrow Articles by Mitsuhashi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ogura, M.
Right arrow Articles by Mitsuhashi, M.
Related Collections
Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 1998;44:2249-2255.)
© 1998 American Association for Clinical Chemistry, Inc.

Molecular Diagnostics and Genetics

RNA chip: quality assessment of RNA by microchannel linear gel electrophoresis in injection-molded plastic chips

Mieko Ogura1, Yoichi Agata2, Kenji Watanabe2, Randy M. McCormick3, Yohei Hamaguchi4, Yoshimasa Aso4, and Masato Mitsuhashi1,4,a

1 Hitachi Chemical Research Center, Irvine, CA 92612.

2 Goshomiya Works, Hitachi Chemical Company, Ltd., Shimodate 308, Japan.

3 Soane BioSciences, Hayward, CA 94545-3716.

4 Department of Pathology, University of California, Irvine, CA 92697.
a Address correspondence to this author at: 1003 Health Sciences Road West, Irvine, CA 92612. Fax 714-725-2727; e-mail mmitsuha{at}uci.edu.

Two major components of rRNA (18S and 28S rRNA) were separated by electrophoresis in injection-molded acrylic chips with a microchannel 100 µm in width, 40 µm in depth, and with 1 cm of separation distance. Microchannels were filled with 4 g/L hydroxypropylmethylcellulose as sieving polymer and 5 mg/L ethidium bromide for RNA staining. The fluorescent signals were detected by a fluorescent microscope equipped with a photometer and 590 nm emission filter. The assay is rapid (<3 min), reproducible, RNase-free, and requires only 1–2 µL of sample. The detection limit was ~10 mg/L (10 ng/µL), 100-fold lower than that for conventional agarose gel electrophoresis. Because only 0.1 nL of the loaded sample was used for electrophoresis, the detectable peaks of rRNA in the separation were derived from less RNA than in a single cell. Because the quality of RNA is critical for RNA-related diagnostic tests, disposable plastic chips will be useful for quality assessment of RNA.




The following articles in journals at HighWire Press have cited this article:


Home page
 DiabetesHome page
L. Van Lommel, K. Janssens, R. Quintens, K. Tsukamoto, D. Vander Mierde, K. Lemaire, C. Denef, J.-C. Jonas, G. Martens, D. Pipeleers, et al.
Probe-Independent and Direct Quantification of Insulin mRNA and Growth Hormone mRNA in Enriched Cell Preparations
Diabetes, December 1, 2006; 55(12): 3214 - 3220.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. E. DiSanto, R. Stein, S. Chang, J. A. Hypolite, Y. Zheng, S. Zderic, A. J. Wein, and S. Chacko
Alteration in expression of myosin isoforms in detrusor smooth muscle following bladder outlet obstruction
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1397 - C1410.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Sobczak and W. J. Krzyzosiak
RNA structure analysis assisted by capillary electrophoresis
Nucleic Acids Res., November 15, 2002; 30(22): e124 - e124.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Pourahmadi, M. Taylor, G. Kovacs, K. Lloyd, S. Sakai, T. Schafer, B. Helton, L. Western, S. Zaner, J. Ching, et al.
Toward a Rapid, Integrated, and Fully Automated DNA Diagnostic Assay for Chlamydia trachomatis and Neisseria gonorrhoeae
Clin. Chem., September 1, 2000; 46(9): 1511 - 1513.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
Y.-H. Chen, W.-C. Wang, K.-C. Young, T.-T. Chang, and S.-H. Chen
Plastic Microchip Electrophoresis for Analysis of PCR Products of Hepatitis C Virus
Clin. Chem., November 1, 1999; 45(11): 1938 - 1943.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Association for Clinical Chemistry.