| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Enzymes and Protein Markers |
1
Osteometer BioTech A/S, Osteopark, Herlev Hovedgade 207, DK-2730 Herlev, Denmark.
2
Center for Clinical and Basic Research, Ballerup Byvej
222, DK-2750 Ballerup, Denmark.
a Author for correspondence. Fax 45 44 94 89 40;
We have developed a two-site ELISA for measurement in serum of bone-related degradation products derived from C-terminal telopeptides of type I collagen. The assay is based on the application of two highly specific monoclonal antibodies against the amino acid sequence of AHD-ß-GGR, where the aspartic acid residue (D) is ß-isomerized. In a one-step incubation procedure, the degradation products containing cross-linked diisomerized EKAHD-ß-GGR peptides are captured by a biotinylated antibody and a peroxidase-conjugated antibody. The generated complex is then bound to the streptavidin surface via the biotin conjugate. Desalted urinary antigens are used for standardization, and parallelism is observed with serum samples. Results are obtained in <2.5 h, and both inter- and intraassay imprecision are <8%. The serum CrossLaps(TM) concentration was 1748 ± 740 pmol/L (mean ± SD) in premenopausal women (n = 65) and 2952 ± 1325 pmol/L in a group of healthy postmenopausal women (n = 169). The Serum CrossLaps One Step ELISA was capable of detecting a highly significant (P <0.001) effect of hormone replacement therapy in a retrospective study involving 22 postmenopausal women.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  M Deberg, J-E Dubuc, A Labasse, C Sanchez, E Quettier, A Bosseloir, J-M Crielaard, and Y Henrotin One-year follow-up of Coll2-1, Coll2-1NO2 and myeloperoxydase serum levels in osteoarthritis patients after hip or knee replacement Ann Rheum Dis, February 1, 2008; 67(2): 168 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Standal, N. Abildgaard, U.-M. Fagerli, B. Stordal, O. Hjertner, M. Borset, and A. Sundan HGF inhibits BMP-induced osteoblastogenesis: possible implications for the bone disease of multiple myeloma Blood, April 1, 2007; 109(7): 3024 - 3030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Leger, D. Marinovic, C. Alberti, S. Dorgeret, D. Chevenne, C. L. Marchal, N. Tubiana-Rufi, G. Sebag, and P. Czernichow Lower Bone Mineral Content in Children with Type 1 Diabetes Mellitus Is Linked to Female Sex, Low Insulin-Like Growth Factor Type I Levels, and High Insulin Requirement J. Clin. Endocrinol. Metab., October 1, 2006; 91(10): 3947 - 3953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J I Kuiper, J H A M Verbeek, V Everts, J P Straub, and M H W Frings-Dresen Serum markers of collagen metabolism: construction workers compared to sedentary workers Occup. Environ. Med., June 1, 2005; 62(6): 363 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Fohr, C. R. Dunstan, and M. J. Seibel Markers of Bone Remodeling in Metastatic Bone Disease J. Clin. Endocrinol. Metab., November 1, 2003; 88(11): 5059 - 5075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Szulc, B. Claustrat, F. Marchand, and P. D. Delmas Increased Risk of Falls and Increased Bone Resorption in Elderly Men with Partial Androgen Deficiency: The MINOS Study J. Clin. Endocrinol. Metab., November 1, 2003; 88(11): 5240 - 5247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U Mouritzen, S Christgau, H-J Lehmann, L B Tanko, and C Christiansen Cartilage turnover assessed with a newly developed assay measuring collagen type II degradation products: influence of age, sex, menopause, hormone replacement therapy, and body mass index Ann Rheum Dis, April 1, 2003; 62(4): 332 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Crofton, N. Evans, and R. Stephen Serum CrossLaps Compared with Other Markers of Bone Turnover in Severely Malnourished Children before and after Refeeding Clin. Chem., January 1, 2003; 49(1): 192 - 195. [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Lehtonen-Veromaa, T. T Mottonen, I. O Nuotio, K. M. Irjala, A. E Leino, and J. S. Viikari Vitamin D and attainment of peak bone mass among peripubertal Finnish girls: a 3-y prospective study Am. J. Clinical Nutrition, December 1, 2002; 76(6): 1446 - 1453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Harper-Wynne, G. Ross, N. Sacks, J. Salter, N. Nasiri, J. Iqbal, R. A'Hern, and M. Dowsett Effects of the Aromatase Inhibitor Letrozole on Normal Breast Epithelial Cell Proliferation and Metabolic Indices in Postmenopausal Women: A Pilot Study for Breast Cancer Prevention Cancer Epidemiol. Biomarkers Prev., July 1, 2002; 11(7): 614 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H J Lehmann, U Mouritzen, S Christgau, P A C Cloos, and C Christiansen Effect of bisphosphonates on cartilage turnover assessed with a newly developed assay for collagen type II degradation products Ann Rheum Dis, June 1, 2002; 61(6): 530 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Crofton, N. Evans, M. R.H. Taylor, and C. V. Holland Serum CrossLaps: Pediatric Reference Intervals from Birth to 19 Years of Age Clin. Chem., April 1, 2002; 48(4): 671 - 673. [Full Text] [PDF]
|
|
|
|
 |
|
 V. Parikka, P. Lehenkari, M.-L. Sassi, J. Halleen, J. Risteli, P. Harkonen, and H. K. Vaananen Estrogen Reduces the Depth of Resorption Pits by Disturbing the Organic Bone Matrix Degradation Activity of Mature Osteoclasts Endocrinology, December 1, 2001; 142(12): 5371 - 5378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Paglia, S. Dionisi, S. De Geronimo, R. Rosso, E. Romagnoli, N. Raejentroph, A. Ragno, M. Celi, J. Pepe, E. D'Erasmo, et al. Biomarkers of Bone Turnover after a Short Period of Steroid Therapy in Elderly Men Clin. Chem., July 1, 2001; 47(7): 1314 - 1316. [Full Text] [PDF]
|
|
|
|
|
|
P Garnero, M Piperno, E Gineyts, S Christgau, P D Delmas, and E Vignon Cross sectional evaluation of biochemical markers of bone, cartilage, and synovial tissue metabolism in patients with knee osteoarthritis: relations with disease activity and joint damage Ann Rheum Dis, June 1, 2001; 60(6): 619 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Garnero, O. Borel, and P. D. Delmas Evaluation of a Fully Automated Serum Assay for C-Terminal Cross-Linking Telopeptide of Type I Collagen in Osteoporosis Clin. Chem., April 1, 2001; 47(4): 694 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Szulc, F. Munoz, B. Claustrat, P. Garnero, F. Marchand, F. Duboeuf, and P. D. Delmas Bioavailable Estradiol May Be an Important Determinant of Osteoporosis in Men: The MINOS Study J. Clin. Endocrinol. Metab., January 1, 2001; 86(1): 192 - 199. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Greenspan, H. N. Rosen, and R. A. Parker Early Changes in Serum N-Telopeptide and C-Telopeptide Cross-Linked Collagen Type 1 Predict Long-Term Response to Alendronate Therapy in Elderly Women J. Clin. Endocrinol. Metab., October 1, 2000; 85(10): 3537 - 3540. [Abstract] [Full Text]
|
|
|
|
|
|
M. Lehtonen-Veromaa, T. Möttönen, K. Irjala, I. Nuotio, A. Leino, and J. Viikari A 1-Year Prospective Study on the Relationship between Physical Activity, Markers of Bone Metabolism, and Bone Acquisition in Peripubertal Girls J. Clin. Endocrinol. Metab., October 1, 2000; 85(10): 3726 - 3732. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Takahashi, M. Oikawa, and A. Nagano Effect of Age and Menopause on Serum Concentrations of Pentosidine, an Advanced Glycation End Product J. Gerontol. A Biol. Sci. Med. Sci., March 1, 2000; 55(3): 137M - 140. [Abstract] [Full Text]
|
|
|
|
|
|
N. H. Bjarnason and C. Christiansen The Influence of Thinness and Smoking on Bone Loss and Response to Hormone Replacement Therapy in Early Postmenopausal Women J. Clin. Endocrinol. Metab., February 1, 2000; 85(2): 590 - 596. [Abstract] [Full Text]
|
|
|
|
|
|
M. L. Traba, J. A. Calero, C. Mendez-Davila, C. Garcia-Moreno, and C. de la Piedra Different Behaviors of Serum and Urinary CrossLaps ELISA in the Assessment of Bone Resorption in Healthy Girls Clin. Chem., May 1, 1999; 45(5): 682 - 683. [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Lowenson, E. Kim, S. G. Young, and S. Clarke Limited Accumulation of Damaged Proteins in L-Isoaspartyl (D-Aspartyl) O-Methyltransferase-deficient Mice J. Biol. Chem., June 1, 2001; 276(23): 20695 - 20702. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
