Clinical Chemistry
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Clinical Chemistry 44: 2313-2319, 1998;
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(Clinical Chemistry. 1998;44:2313-2319.)
© 1998 American Association for Clinical Chemistry, Inc.

Automation and Analytical Techniques

Automated measurement of serum ferroxidase activity

Ozcan Erel

Department of Clinical Biochemistry, Faculty of Medicine, Research Hospital, Harran University, Sanliurfa, Turkey TR-63100. Fax 414-3151181; e-mail ereloz{at}doruk.net.tr.

A method is described for automated measurement of serum ceruloplasmin ferroxidase activity. In this method, Fe2+ ions are used as the substrate. In addition, a new calibration system without ceruloplasmin is also presented. Optimum assay reaction conditions were determined. Maximal catalytic activity was obtained at 0.45 mol/L acetate buffer, pH 5.8. The reagents and calibrator are stable for at least 6 months. Significant correlations between serum ferroxidase and p-phenylenediamine oxidase activities (r = 0.96; P <0.0001) and copper concentration (r = 0.93; P <0.0001) were found. The range for serum ceruloplasmin ferroxidase activity in healthy persons was 198-1107 U/L, and in patients with bronchial asthma it was 601-1912 U/L.




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