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Clinical Chemistry 44: 2331-2339, 1998;
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(Clinical Chemistry. 1998;44:2331-2339.)
© 1998 American Association for Clinical Chemistry, Inc.

Laboratory Management

Lyophilized bovine hemoglobin as a possible reference material for the determination of hemoglobin derivatives in human blood

Bart H. A. Maas1, Anneke Buursma2, Rob A. J. Ernst1, Anton H. J. Maas3, and Willem G. Zijlstra4,a

1 EURO-TROL b.v., NL-6702 EA Wageningen, The Netherlands.

2 Department of Medical Physiology, University of Groningen, NL-9712 KZ Groningen, The Netherlands.

3 Department of Chemical Technology, Technical University Eindhoven, NL-5600 MB Eindhoven,The Netherlands.

4 Department of Pediatrics, University Hospital, NL-9713 GZ Groningen, The Netherlands.
a Author for correspondence. Fax 31-503-611671; e-mail w.a.kamps{at}med.rug.nl.

We investigated the suitability of a lyophilized bovine hemoglobin (LBH) preparation containing various fractions of oxyhemoglobin (O2Hb), carboxyhemoglobin (COHb), and methemoglobin (MetHb) for quality assessment in multicomponent analysis (MCA) of hemoglobin derivatives. It was demonstrated that a stable preparation of these components after reconstitution yields a hemoglobin solution that is spectrophotometrically equivalent with a fresh bovine hemoglobin solution. The preparation was found to be stable for at least 1 year when it is kept at 2–8 °C and for 1 h after reconstitution. We determined the fractions of O2Hb, COHb, and MetHb of several LBH preparations, using the complete spectra of 480–650 nm with 2-nm intervals and absorptivities as determined for pure LBH solutions. A field trial involving various types of multiwavelength hemoglobin photometers showed the suitability of LBH as a quality-control material. Computer models of the various common multiwavelength hemoglobin photometers may be useful for establishing more accurate target values of LBH preparations for each type of photometer and for studying the importance of the influence of specific factors such as wavelength selection, absorptivity values, and interfering dyes.




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[Abstract] [Full Text] [PDF]


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