Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 44: 2422-2428, 1998;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klein, A.
Right arrow Articles by Michalski, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klein, A.
Right arrow Articles by Michalski, J.-C.
Related Collections
Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 1998;44:2422-2428.)
© 1998 American Association for Clinical Chemistry, Inc.

Molecular Diagnostics and Genetics

Identification of urinary oligosaccharides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

André Klein1,a, Alexandra Lebreton1, Jérome Lemoine2, Jean-Marc Périni1, Philippe Roussel1 and Jean-Claude Michalski2

1 Centre Hospitalier Régional Universitaire de Lille, Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital Calmette, Bd du Professeur Jules Leclercq, 59047 Lille Cedex, France.

2 Laboratoire de Chimie Biologique, CNRS Unité Mixte 111, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Asq Cedex, France.
a Author for correspondence. Fax 33 3 20 53 85 62; e-mail klein{at}lille.inserm.fr.

A new method of urinary oligosaccharides identification by matrix-assisted laser desorption time-of-flight mass spectrometry is presented. The method involves three steps: coupling of the urinary oligosaccharides with 8-aminonaphthalene-1,3,6-trisulfonic acid; fast purification over a porous graphite carbon extraction column; and mass spectrometric analysis. Identification of urinary oligosaccharides is based on the patterns and values of the pseudomolecular ions observed. We report here the patterns in urines from patients with Pompe disease, alpha and beta mannosidoses, galacto-sialidosis, and GM1 gangliosidosis. The protocols described here allowed facile and sensitive identification of the pathognomonic oligosacchariduria present in lysosomal diseases and can be extended to any pathological oligosacchariduria.




The following articles in journals at HighWire Press have cited this article:


Home page
 GlycobiologyHome page
C. Flahaut, J.C. Michalski, T. Danel, M.H. Humbert, and A. Klein
The effects of ethanol on the glycosylation of human transferrin
Glycobiology, March 1, 2003; 13(3): 191 - 198.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Horiuchi, T. Fukushige, T. Saheki, and T. Kanzaki
Improved Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometric Method for Identification of Amino Acid O-Glycosides in Patients with {alpha}-N-Acetylgalactosaminidase Deficiency
Clin. Chem., October 1, 2002; 48(10): 1798 - 1799.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. T. Hepbildikler, R. Sandhoff, M. Kolzer, R. L. Proia, and K. Sandhoff
Physiological Substrates for Human Lysosomal beta -Hexosaminidase S
J. Biol. Chem., January 18, 2002; 277(4): 2562 - 2572.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Association for Clinical Chemistry.