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Enzymes and Protein Markers |
Departments of
1
Laboratory Diagnosis and
2
Surgery, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan.
a Address correspondence to this author at: Department of Laboratory Diagnosis, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan. Fax 81-11-622-7502; e-mail watanabn{at}sapmed.ac.jp.
We established the validity of a quantitative reverse transcription (RT)-PCR assay for the RNA component of human telomerase (hTR), using the TaqMan fluorogenic detection system. Using this assay, we quantified hTR expression in two human pancreatic cancer cell lines, ASPC-1 and MIAPaCa-2. Our results indicated that hTR expression in MIAPaCa-2 was 1.99-fold higher than that in ASPC-1 cells. This TaqMan RT-PCR assay appears to be useful in determining the amount of hTR in clinical specimens.
The following articles in journals at HighWire Press have cited this article:
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  A. Marrone, A. Walne, H. Tamary, Y. Masunari, M. Kirwan, R. Beswick, T. Vulliamy, and I. Dokal Telomerase reverse-transcriptase homozygous mutations in autosomal recessive dyskeratosis congenita and Hoyeraal-Hreidarsson syndrome Blood, December 15, 2007; 110(13): 4198 - 4205. [Abstract] [Full Text] [PDF]
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 T. Nakamura, T. Sakaeda, N. Ohmoto, T. Tamura, N. Aoyama, T. Shirakawa, T. Kamigaki, T. Nakamura, K. I. Kim, S. R. Kim, et al. Real-Time Quantitative Polymerase Chain Reaction for MDR1, MRP1, MRP2, and CYP3A-mRNA Levels in Caco-2 Cell Lines, Human Duodenal Enterocytes, Normal Colorectal Tissues, and Colorectal Adenocarcinomas Drug Metab. Dispos., January 1, 2002; 30(1): 4 - 6. [Abstract] [Full Text] [PDF]
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