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Clinical Chemistry 44: 2446-2452, 1998;
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Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 1998;44:2446-2452.)
© 1998 American Association for Clinical Chemistry, Inc.

Enzymes and Protein Markers

New method to measure telomerase activity by transcription-mediated amplification and hybridization protection assay

Minoru Hirose1,a, Junko Abe-Hashimoto1, Hidetoshi Tahara2, Toshihide Ide2 and Tadashi Yoshimura1

1 Diagnostics Science Laboratory, Chugai Diagnostics Science Co., Ltd., 3-41-8 Takada, Toshima-ku, Tokyo 171-8545, Japan.

2 Department of Cellular and Molecular Biology, Hiroshima University School of Medicine, 1-2-3 Kasumi, Hiroshima 734-0037, Japan.
a Author for correspondence. Fax 81-3-3989-0785; e-mail hirosemnr{at}chugai-pharm.co.jp.

Telomerase is a ribonucleoprotein complex that uses RNA as a template for the addition of telomeric repeats. The development of the telomeric repeat amplification protocol (TRAP), a sensitive PCR-based assay, has facilitated the detection of telomerase activity in small tissue and tumor samples. Telomerase activity is expected to be a new diagnostic and prognostic marker of human cancer. In this study, we applied a non-PCR-based transcription-mediated amplification (TMA) and hybridization protection assay (HPA) to the measurement of telomerase activity by modification of both primers in TMA. We demonstrated that the modified TMA can detect and measure telomerase activity. TMA/HPA is as sensitive and reproducible as conventional TRAP, but is both faster and easier to perform. Furthermore, we found that TMA/HPA was influenced minimally by TRAP inhibitors that may come from clinical samples. TMA/HPA, which is easy, rapid, and applicable to a high-throughput format, should be clinically useful for the detection and monitoring of telomerase activity.




The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
Y. Nakamura, M. Hirose, H. Matsuo, N. Tsuyama, K. Kamisango, and T. Ide
Simple, Rapid, Quantitative, and Sensitive Detection of Telomere Repeats in Cell Lysate by a Hybridization Protection Assay
Clin. Chem., October 1, 1999; 45(10): 1718 - 1724.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
Y. M. D. Lo
Quantitative Assays for Telomerase: Means for Studying the End
Clin. Chem., December 1, 1998; 44(12): 2399 - 2400.
[Full Text] [PDF]


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