Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 44: 2490-2498, 1998;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (67)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leary, E. T.
Right arrow Articles by Havel, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leary, E. T.
Right arrow Articles by Havel, R. J.
Related Collections
Right arrow Lipids, Lipoproteins, and Cardiovascular Risk Factors
(Clinical Chemistry. 1998;44:2490-2498.)
© 1998 American Association for Clinical Chemistry, Inc.

Lipids and Lipoproteins

Evaluation of an immunoseparation method for quantitative measurement of remnant-like particle-cholesterol in serum and plasma

Elizabeth Teng Leary1,a, Tao Wang2, Daniel J. Baker1, Donald D. Cilla2, Jianhua Zhong2, G. Russell Warnick1, Katsuyuki Nakajima2 and Richard J. Havel3

1 Pacific Biometrics, Inc., 220 West Harrison Street, Seattle, WA 98119.

2 Otsuka America Pharmaceutical, Inc., Rockville, MD 20850.

3 Cardiovascular Research Institute, University of California, San Francisco, CA 94143.
a Author for correspondence. Fax 206-298-9838; e-mail ETL{at}PACBIO.COM.

Substantial evidence indicates that triglyceride-rich lipoprotein remnants are atherogenic. Additional research has, however, been limited by available methods for separation and quantification of remnants. We have evaluated an immunoseparation assay developed to measure cholesterol in remnant-like particles (RLP-C). This method uses monoclonal antibodies to human apolipoproteins B-100 and A-I to remove most of the apolipoprotein B-100-containing lipoproteins (namely LDL and nascent VLDL) and apolipoprotein A-I-containing lipoproteins (namely chylomicrons and HDL), leaving behind a fraction of triglyceride-rich lipoproteins, including chylomicron and VLDL remnants, both of which are enriched in apolipoprotein E. Cholesterol in the unbound fraction is measured with a sensitive enzymatic assay. The RLP-C concentration was highly correlated with total triglyceride-rich lipoproteins (sum of VLDL-cholesterol and IDL-cholesterol) separated by ultracentrifugation and by polyacrylamide gel electrophoresis (r = 0.86 and 0.76, respectively). The within-run and run-to-run imprecision (CV) of the assay was ~6% and 10%, respectively. The assay was not affected by hemoglobin up to 5000 mg/L (500 mg/dL), bilirubin up to 342 mmol/L (20 mg/dL), glucose up to 67 mmol/L (1200 mg/dL), or ascorbic acid up to 170 mmol/L (3.0 mg/dL). In 726 subjects (men, n = 364; women, n = 362) in the US, the 75th percentiles of RLP-C concentration were 0.17 mmol/L (6.6 mg/dL) and 0.23 mmol/L (8.8 mg/dL) in sera obtained after overnight fasting or randomly, respectively. A group of 151 patients from nine US centers and one Canadian center with coronary artery atherosclerosis established by angiography had higher median RLP-C concentrations than 302 gender- and age-matched controls (P <0.05). We conclude that the RLP-C assay compares favorably to ultracentrifugation and electrophoresis and provides a convenient and economical approach to measure triglyceride-rich lipoprotein remnants in routine clinical laboratories.




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Lipid Res.Home page
X.-Y. Zheng and L. Liu
Remnant-like lipoprotein particles impair endothelial function: direct and indirect effects on nitric oxide synthase
J. Lipid Res., August 1, 2007; 48(8): 1673 - 1680.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
B. C.H. Kwan, F. Kronenberg, S. Beddhu, and A. K. Cheung
Lipoprotein Metabolism and Lipid Management in Chronic Kidney Disease
J. Am. Soc. Nephrol., April 1, 2007; 18(4): 1246 - 1261.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
Y. Iwasaki, H. Matsuyama, and N. Nakashima
Improved Specificity of a New Homogeneous Assay for LDL-Cholesterol in Serum with Abnormal Lipoproteins
Clin. Chem., May 1, 2006; 52(5): 886 - 888.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
E. A. Brinton, M. N. Nanjee, and P. N. Hopkins
Triglyceride-rich lipoprotein remnant levels and metabolism: Time to adopt these orphan risk factors?
J. Am. Coll. Cardiol., June 16, 2004; 43(12): 2233 - 2235.
[Full Text] [PDF]

Home page
 CirculationHome page
T.B. Twickler, G.M. Dallinga-Thie, J.S. Cohn, and M.J. Chapman
Elevated Remnant-Like Particle Cholesterol Concentration: A Characteristic Feature of the Atherogenic Lipoprotein Phenotype
Circulation, April 27, 2004; 109(16): 1918 - 1925.
[Full Text] [PDF]

Home page
 J. Lipid Res.Home page
Y. Hirowatari, H. Yoshida, H. Kurosawa, K.-i. Doumitu, and N. Tada
Measurement of cholesterol of major serum lipoprotein classes by anion-exchange HPLC with perchlorate ion-containing eluent
J. Lipid Res., July 1, 2003; 44(7): 1404 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
References
Circulation, December 17, 2002; 106(25): 3373 - 3421.
[Full Text]

Home page
 CirculationHome page
P. R. W. de Sauvage Nolting, M. B. Twickler, G. M. Dallinga-Thie, R. J.A. Buirma, B. A. Hutten, J. J.P. Kastelein, and for the Examination of Probands and Relatives in S
Elevated Remnant-Like Particles in Heterozygous Familial Hypercholesterolemia and Response to Statin Therapy
Circulation, August 13, 2002; 106(7): 788 - 792.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
D. C. Chan, G. F. Watts, P. H. R. Barrett, I. J. Martins, A. P. James, J. C. L. Mamo, T. A. Mori, and T. G. Redgrave
Effect of atorvastatin on chylomicron remnant metabolism in visceral obesity: a study employing a new stable isotope breath test
J. Lipid Res., May 1, 2002; 43(5): 706 - 712.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
K. Kishi, K. Ochiai, Y. Ohta, Y. Uemura, K. Kanatani, K. Nakajima, and M. Nakamura
Highly Sensitive Cholesterol Assay with Enzymatic Cycling Applied to Measurement of Remnant Lipoprotein-Cholesterol in Serum
Clin. Chem., May 1, 2002; 48(5): 737 - 741.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
E. Campos, L. Kotite, P. Blanche, Y. Mitsugi, P. H. Frost, U. Masharani, R. M. Krauss, and R. J. Havel
Properties of triglyceride-rich and cholesterol-rich lipoproteins in the remnant-like particle fraction of human blood plasma
J. Lipid Res., March 1, 2002; 43(3): 365 - 374.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
I. Jialal and S. Devaraj
Remnant Lipoproteins: Measurement and Clinical Significance
Clin. Chem., February 1, 2002; 48(2): 217 - 219.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Nauck, G. R. Warnick, and N. Rifai
Methods for Measurement of LDL-Cholesterol: A Critical Assessment of Direct Measurement by Homogeneous Assays versus Calculation
Clin. Chem., February 1, 2002; 48(2): 236 - 254.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. C. Chan, G. F. Watts, P. H. Barrett, J. C.L. Mamo, and T. G. Redgrave
Markers of Triglyceride-rich Lipoprotein Remnant Metabolism in Visceral Obesity
Clin. Chem., February 1, 2002; 48(2): 278 - 283.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
H.-S. Kim, F. Abbasi, C. Lamendola, T. McLaughlin, and G. M Reaven
Effect of insulin resistance on postprandial elevations of remnant lipoprotein concentrations in postmenopausal women
Am. J. Clinical Nutrition, November 1, 2001; 74(5): 592 - 595.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. Koutsari, F. Karpe, S. M. Humphreys, K. N. Frayn, and A. E. Hardman
Exercise Prevents the Accumulation of Triglyceride-Rich Lipoproteins and Their Remnants Seen When Changing to a High-Carbohydrate Diet
Arterioscler. Thromb. Vasc. Biol., September 1, 2001; 21(9): 1520 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
T. G. Redgrave, G. F. Watts, I. J. Martins, P. H. R. Barrett, J. C. L. Mamo, S. B. Dimmitt, and A. D. Marais
Chylomicron remnant metabolism in familial dyslipidemias studied with a remnant-like emulsion breath test
J. Lipid Res., May 1, 2001; 42(5): 710 - 715.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Th. B. Twickler, G. M. Dallinga-Thie, H. W. de Valk, P. C. N. J. Schreuder, H. Jansen, M. C. Cabezas, and D. W. Erkelens
High Dose of Simvastatin Normalizes Postprandial Remnant-Like Particle Response in Patients With Heterozygous Familial Hypercholesterolemia
Arterioscler. Thromb. Vasc. Biol., November 1, 2000; 20(11): 2422 - 2427.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
C. Marcoux, P. N. Hopkins, T. Wang, E. T. Leary, K. Nakajima, J. Davignon, and J. S. Cohn
Remnant-like particle cholesterol and triglyceride levels of hypertriglyceridemic patients in the fed and fasted state
J. Lipid Res., September 1, 2000; 41(9): 1428 - 1436.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
D. M. Waterworth, J. A. Hubacek, J. Pitha, J. Kovar, R. Poledne, S. E. Humphries, and P. J. Talmud
Plasma levels of remnant particles are determined in part by variation in the APOC3 gene insulin response element and the APOCI;-APOE cluster
J. Lipid Res., July 1, 2000; 41(7): 1103 - 1109.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
S. Hirany, D. O'Byrne, S. Devaraj, and I. Jialal
Remnant-like Particle-Cholesterol Concentrations in Patients with Type 2 Diabetes Mellitus and End-Stage Renal Disease
Clin. Chem., May 1, 2000; 46(5): 667 - 672.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
E. J Parks and M. K Hellerstein
Carbohydrate-induced hypertriacylglycerolemia: historical perspective and review of biological mechanisms1
Am. J. Clinical Nutrition, February 1, 2000; 71(2): 412 - 433.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Wang, K. Nakajima, E. T. Leary, G. R. Warnick, J. S. Cohn, P. N. Hopkins, L. L. Wu, D. D. Cilla, J. Zhong, and R. J. Havel
Ratio of Remnant-like Particle-Cholesterol to Serum Total Triglycerides Is an Effective Alternative to Ultracentrifugal and Electrophoretic Methods in the Diagnosis of Familial Type III Hyperlipoproteinemia
Clin. Chem., November 1, 1999; 45(11): 1981 - 1987.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. S. Cohn, C. Marcoux, and J. Davignon
Detection, Quantification, and Characterization of Potentially Atherogenic Triglyceride-Rich Remnant Lipoproteins
Arterioscler. Thromb. Vasc. Biol., October 1, 1999; 19(10): 2474 - 2486.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. ANDO, T. SANAKA, and H. NIHEI
Eicosapentanoic Acid Reduces Plasma Levels of Remnant Lipoproteins and Prevents in Vivo Peroxidation of LDL in Dialysis Patients
J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2177 - 2184.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Association for Clinical Chemistry.