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Clinical Chemistry 44: 2499-2505, 1998;
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Right arrow Lipids, Lipoproteins, and Cardiovascular Risk Factors
(Clinical Chemistry. 1998;44:2499-2505.)
© 1998 American Association for Clinical Chemistry, Inc.

Lipids and Lipoproteins

Comparison of two homogeneous assays with a precipitation method and an ultracentrifugation method for the measurement of HDL-cholesterol

Maria-Luisa Arranz-Peñaa, Juan Tasende-Mata and Francisco J. Martin-Gil

a Address correspondence to this author at: Laboratorio de Análisis Clínicos, Sección de Bioquímica, Hospital Universitario "Del Río Hortega", calle Cardenal Torquemada, s/n, 47010 Valladolid, Spain. Fax 34-983-331566; e-mail marisa.arranz{at}mx2.redestb.es.

We report on the performance of four HDL-cholesterol assays: a homogeneous method based on polyethylene glycol-modified enzymes/{alpha}-cyclodextrin sulfate (PEGME; Kyowa); a homogeneous method based on polyanion–polymer/detergent (PPD; Daiichi); the usual precipitation method with phosphotungstic acid/MgCl2 (PTA); and an ultracentrifugation (UC) procedure. The homogeneous HDL-cholesterol assays (performed with automated analyzers) were precise and correlated well with the PTA and UC assays. The specificity and accuracy of the PEGME method were better than those of the PPD method.




The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
Z. Li, R. Yang, G. Xu, and T. Xia
Serum Lipid Concentrations and Prevalence of Dyslipidemia in a Large Professional Population in Beijing
Clin. Chem., January 1, 2005; 51(1): 144 - 150.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
G. R. Warnick, M. Nauck, and N. Rifai
Evolution of Methods for Measurement of HDL-Cholesterol: From Ultracentrifugation to Homogeneous Assays
Clin. Chem., September 1, 2001; 47(9): 1579 - 1596.
[Abstract] [Full Text] [PDF]


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