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Clinical Chemistry 44: 221-225, 1998;
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Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 1998;44:221-225.)
© 1998 American Association for Clinical Chemistry, Inc.

Enzymes and Protein Markers

Improved method for measuring tartrate-resistant acid phosphatase activity in serum

Mamoru Nakanishi1,3,a, Kousei Yoh2, Kagehiro Uchida3, Souji Maruo2, and Akira Matsuoka1

1 Departments of Clinical Pathology and
2 Orthopedic Surgery, Hyogo College of Medicine, 1–1 Mukogawa-cho, Nishinomiya, Hyogo, Japan.

3 FALCO Biosystems Co., 17–1 Nishiarami Tai, Kumiyama-cho, Kuze-gun, Kyoto, Japan.
a Author for correspondence. Fax 0798-45-6317.

We describe an improved method for the kinetic measurement of tartrate-resistant acid phosphatase (TrACP; EC 3.1.3.2) activity in serum. Of the TrACP derived from erythrocytes, platelets, and macrophages (osteoclasts and others), that from the first two sources is also resistant to fluoride, whereas skeletal TrACP is sensitive to fluoride. Thus, osteoclast-derived TrACP can be measured specifically by exploiting its sensitivity to fluoride. We measured the activity of tartrate-resistant and fluoride-sensitive acid phosphatase (TrFsACP) by using 2,6-dichloro-4-acetylphenyl phosphate as substrate at pH 6.2. The activity of TrFsACP in serum was increased by adding hexadimethrine bromide (Polybrene) to the reaction mixture. This method was not influenced by hemolysis with hemoglobin concentrations as great as 0.9 g/L. The mean ± SD values of TrFsACP activity by this method were 20.4 ± 2.8 and 16.4 ± 2.3 U/L for young (ages 20–29 years) men (n = 34) and women (n = 50), respectively. The highest mean TrFsACP activity was found among children younger than 15 years, followed by that in elderly subjects (older than 60).




The following articles in journals at HighWire Press have cited this article:


Home page
 Mol. Pathol.Home page
H Bull, P G Murray, D Thomas, A M Fraser, and P N Nelson
Acid phosphatases
Mol. Pathol., April 1, 2002; 55(2): 65 - 72.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
J. M. Halleen, S. L. Alatalo, A. J. Janckila, H. W. Woitge, M. J. Seibel, and H. K. Vaananen
Serum Tartrate-resistant Acid Phosphatase 5b Is a Specific and Sensitive Marker of Bone Resorption
Clin. Chem., March 1, 2001; 47(3): 597 - 600.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Nakanishi, K. Yoh, T. Miura, T. Ohasi, S. K. Rai, and K. Uchida
Development of a Kinetic Assay for Band 5b Tartrate-resistant Acid Phosphatase Activity in Serum
Clin. Chem., April 1, 2000; 46(4): 469 - 473.
[Abstract] [Full Text] [PDF]


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