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Clinical Chemistry 44: 244-249, 1998;
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Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 1998;44:244-249.)
© 1998 American Association for Clinical Chemistry, Inc.

Endocrinology and Metabolism

Rapid, convenient radioimmunoassay of estrone sulfate

Girish N. Ranadive1,a, Jehangir S. Mistry1, Kalyani Damodaran1, M. Javad Khosravi2, Anastasia Diamandi2, Terry Gimpel3, V. Daniel Castracane3, S. Patel4, and Frank Z. Stanczyk4

1 Diagnostic Systems Laboratories, 445 Medical Center Blvd., Webster, TX 77598-4217.

2 Diagnostic Systems Laboratories, 600 University Ave., Room 653, Toronto, Ontario M5G1X5, Canada.

3 Texas Tech University, Health Science Center, Department of Obstetrics and Gynecology, 1400 Wallace Rd., Amarillo, TX 79106-1797.

4 University of Southern California School of Medicine, Women and Children's Hospital, 1240 N. Mission Rd., Los Angeles, CA 90033.
a Author for correspondence. Fax (281) 554-4220.

We developed a specific, simple, and rapid RIA for the direct quantification of estrone sulfate (E1S) and established its performance characteristics. The assay has a dynamic range of 0.05–90 µg/L with a detection limit of 0.009 µg/L. Intraassay CVs were 9.2%, 4.5%, and 4.6% at 0.35, 9.0, and 60 µg/L, respectively. Interassay CVs were 8.8%, 5.1%, and 5.5% at 0.076, 0.5, and 12 µg/L, respectively. Linearity of dilution studies showed values of 80–105% of expected, and recovery of E1S added to serum samples ranged from 82% to 102%. Cross-reactivities with structurally related estrogens were <5%. When compared with a conventional assay (involving hydrolysis of E1S and indirect measurement of estrone), the present RIA showed excellent correlation (r = 0.99, slope = 1.54, Sy||x = 2.14, n = 71). Mean E1S concentrations measured with this RIA for normal men (n = 20) and women in follicular (n = 20) and luteal (n = 25) phases of their menstrual cycle were 0.96, 0.96, and 1.74 µg/L, respectively. Mean E1S concentrations for oral contraceptive users (n = 20) and postmenopausal women without hormone replacement therapy (n = 21) or on hormone replacement therapy (n = 22) were 0.74, 0.13, and 2.56 µg/L, respectively. Serum concentrations of E1S in pregnant women in their first (n = 14), second (n = 17), and third (n = 15) trimesters were 20, 66, and 105 µg/L, respectively. Availability of this simple RIA should provide a useful tool for the assessment of estrogen status in women.




The following articles in journals at HighWire Press have cited this article:


Home page
 J EndocrinolHome page
C. Dabrosin
Increased extracellular local levels of estradiol in normal breast in vivo during the luteal phase of the menstrual cycle
J. Endocrinol., October 1, 2005; 187(1): 103 - 108.
[Abstract] [Full Text] [PDF]


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