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Clinical Chemistry 44: 287-292, 1998;
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Right arrow Drug Monitoring and Toxicology
(Clinical Chemistry. 1998;44:287-292.)
© 1998 American Association for Clinical Chemistry, Inc.

Drug Monitoring and Toxicology

Effective GC-MS procedure for detecting iso-LSD in urine after base-catalyzed conversion to LSD

Edward D. Clarkson, David Lesser, and Buddha D. Paul1,a

1 Division of Forensic Toxicology, Office of the Armed Forces Medical Examiner, Armed Forces Institute of Pathology, Rockville, MD 20850.
a Address correspondence to this author at: Forensic Toxicology, AFIP Annex, 1413 Research Blvd., Rockville, MD 20850. Fax 301-319-0628; e-mail paul{at}email.afip.osd.mil.

A sensitive method is described to detect isolysergic acid diethylamide (iso-LSD) in urine. The compound was extracted from urine and converted to a C-8 carbanion by sodium ethoxide in ethanol. Protonation of the carbanion by water selectively produced LSD. The conversion of iso-LSD to LSD was almost quantitative (98%). The product was purified by solid-phase fractionation and acid–base separation techniques. The trimethylsilyl derivative of LSD was detected by a gas chro-matography–mass spectrometry method. The overall recovery of the procedure was approximately 69%. Quantification of iso-LSD was linear over the concentration range 50–2000 ng/L. In specimen analysis, iso-LSD was detected when the LSD concentration was below the limit of the detection (50 ng/L) of the procedure. Because iso-LSD is a byproduct of illicit preparation of LSD, presence of iso-LSD in urine is an indication of LSD use.




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