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Clinical Chemistry 44: 311-316, 1998;
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(Clinical Chemistry. 1998;44:311-316.)
© 1998 American Association for Clinical Chemistry, Inc.

Automation and Analytical Techniques

Enzyme conversion immunoassay for determining total homocysteine in plasma or serum

Frank Frantzen1,a, Arne Ludvig Faaren1, Ingrid Alfheim2, and Arne Kristian Nordhei1

1 Axis Nord, P.O. Box 6073, N-8018 Mørkved, Norway.

2 Axis Biochemicals ASA, P.O. Box 2123, Grünerløkka, N-0505 Oslo, Norway.
a Author for correspondence.

A rapid and precise immunoassay for quantification of total homocysteine in blood samples is presented. The method avoids the use of radioisotopes and chromatographic separations and relies on enzymatic conversion of homocysteine to S-adenosyl-L-homocysteine, followed by quantification of S-adenosyl-L-homocysteine by an enzyme-linked immunoassay in microtiter format. The within- and between-assay imprecision is <6% and 8%, respectively, and results by the method show good correlation with those by HPLC. Including controls and calibrators in duplicates, 82 samples can be analyzed within 2.5 h. The procedure can be fully automated.




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