Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 44: 502-508, 1998;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Küllertz, G.
Right arrow Articles by Fischer, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Küllertz, G.
Right arrow Articles by Fischer, G.
Related Collections
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 1998;44:502-508.)
© 1998 American Association for Clinical Chemistry, Inc.

Enzymes and Protein Markers

Semiautomated microtiter plate assay for monitoring peptidylprolyl cis/trans isomerase activity in normal and pathological human sera

Gerhard Küllertz1,a, Sabine Lüthe2, and Gunter Fischer1

1 Max-Planck Research Unit, Enzymology of Protein Folding, Kurt-Mothes Str. 3, D-06120 Halle/Saale, Germany.

2 Martin-Luther-University Halle-Wittenberg, Institut for Clinical Chemistry and Pathobiochemistry, Madgeburger Str., D-06112 Halle/Saale, Germany.
a Author for correspondence. Fax +49–345 5511972;

An UV/VIS spectrophotometric assay technique was developed that was able to routinely monitor peptidylprolyl cis/trans isomerase (PPIase) activity of biological fluids in 96-well microtiter plates. The assay, based on monitoring the cis-to-trans isomerization of succinyl-Phe-cisPro-Phe-4-nitroanilide as substrate in a chymotrypsin-coupled reaction, yields a throughput of 96 samples per 30 min. The assay's capacity was exemplified by dealing with the PPIase activity in several normal and pathological human sera. Reference values of 151 healthy subjects (83 females, 69 males, 17 to 60 years old) were found to possess significant sex-specific differences. PPIase activity factor K of the sera was significantly greater in males (5th, 50th, 95th percentiles: 17, 36, 55 K) than females (14, 30, 48 K). PPIase activities of sera from healthy donors (n = 151) were significantly higher (Mann–Whitney rank-sum test P <0.0001) than those of patients (n = 47). PPIase activity in serum samples stored at 4 °C was stable for at least 20 h.




The following articles in journals at HighWire Press have cited this article:


Home page
 J Biomol ScreenHome page
T. Mori, S. Itami, T. Yanagi, Y. Tatara, M. Takamiya, and T. Uchida
Use of a Real-Time Fluorescence Monitoring System for High-Throughput Screening for Prolyl Isomerase Inhibitors
J Biomol Screen, April 1, 2009; 14(4): 419 - 424.
[Abstract] [PDF]

Home page
 Biol. Reprod.Home page
A. N. Santos, S. Körber, G. Küllertz, G. Fischer, and B. Fischer
Oxygen Stress Increases Prolyl cis/trans Isomerase Activity and Expression of Cyclophilin 18 in Rabbit Blastocysts
Biol Reprod, January 1, 2000; 62(1): 1 - 7.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. Metzner, G. Stoller, K. P. Rucknagel, K. P. Lu, G. Fischer, M. Luckner, and G. Kullertz
Functional Replacement of the Essential ESS1 in Yeast by the Plant Parvulin DlPar13
J. Biol. Chem., April 20, 2001; 276(17): 13524 - 13529.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Association for Clinical Chemistry.