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Enzymes and Protein Markers |
1
Novadex, Inc., San Diego, CA 92121.
2
University of CaliforniaSan Diego Medical Center,
Division of Rheumatology, San Diego, CA 92103.
3
University of Nebraska Medical Center, Department
of Internal Medicine, Omaha, NE 68198.
4
Metra Biosystems, Inc., 265 N. Whisman Rd.,
Mountain View, CA 94043.
a Author for correspondence. Fax 650-903-9500; e-mail jvisor{at}metrabio.com.
Chondrex, a major secretory protein of human chondrocytes and synovial fibroblasts, is increased in serum of patients with joint and cartilage disease. We have developed a sandwich-type ELISA for quantifying chondrex in serum. The interassay CVs were 2.83.7% and the average within-run and total CVs were 3.6% and 5.4%, respectively. The limit of detectability by linear dilution was 20 µg/L, recovery upon dilution was 102% ± 5%, and analytical recovery (of added analyte) was 98% ± 11%. The reference interval (central 90% interval) for chondrex in healthy adults was 2595 µg/L. Chondrex values for patients with active rheumatoid arthritis or osteoarthritis were significantly greater than in healthy adults, inactive rheumatoid arthritis patients, and diabetes patients (P <0.05). In patients treated with disease-modifying antirheumatic drug therapy, decreasing chondrex values reflected the clinical improvement observed in responders, whereas the values were maintained or increased in nonresponders. In conclusion, chondrex may be a useful marker in the clinical investigation of arthritis.
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