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Lipids and Lipoproteins |
-cyclodextrin sulfate
1
Department of Central Laboratory, Kumamoto University Hospital, 1-1-1, Honjo, Kumamoto 860, Japan.
2
Faculty of Pharmaceutical Sciences, Kumamoto University,
5–1, Oe-honmachi, Kumamoto 862, Japan.
3
Department of Laboratory Medicine, Kumamoto University,
School of Medicine, 1-1-1, Honjo, Kumamoto 860, Japan.
a Author for correspondence. Fax (81) 96-362-7540;
We have developed a fully automated method for measuring
LDL-cholesterol (LDL-C) in human serum without the need for prior
separation, using a nonionic surfactant,
polyoxyethylene–polyoxypropylene block copolyether (POE-POP), and a
sodium salt of sulfated cyclic maltohexaose, -cyclodextrin sulfate.
Of the surfactants tested, POE-POP with a higher molecular mass of the
POP block and a greater hydrophobicity reduced the reactivity of
cholesterol in lipoprotein fractions; the reactivity in descending
order was LDL 
VLDL > chylomicron 
HDL. Gel
filtration chromatographic studies revealed that POE-POP removed lipids
selectively from the LDL fraction and allowed them to participate in
the cholesterol esterase–cholesterol oxidase coupling reaction system.
By contrast, -cyclodextrin sulfate reduced the reactivity of
cholesterol, especially in chylomicrons and VLDL. A combination of
POE-POP with -cyclodextrin sulfate provided the required selectivity
for the determination of LDL-C in serum in the presence of magnesium
ions and a small amount of dextran sulfate without precipitating
lipoprotein aggregates. There was a good correlation between the
results of LDL-C assayed by the proposed method and the
beta-quantification reference method involving 161 sera with
triglyceride concentrations ranging from 0.3 to 22.6 mmol/L.
The following articles in journals at HighWire Press have cited this article:
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  K. Miyauchi, N. Kayahara, M. Ishigami, H. Kuwata, H. Mori, H. Sugiuchi, T. Irie, A. Tanaka, S. Yamashita, and T. Yamamura Development of a Homogeneous Assay to Measure Remnant Lipoprotein Cholesterol Clin. Chem., December 1, 2007; 53(12): 2128 - 2135. [Abstract] [Full Text] [PDF]
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W. G. Miller, P. P. Waymack, F. P. Anderson, S. F. Ethridge, and E. C. Jayne Performance of Four Homogeneous Direct Methods for LDL-Cholesterol Clin. Chem., March 1, 2002; 48(3): 489 - 498. [Abstract] [Full Text] [PDF]
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M. Nauck, G. R. Warnick, and N. Rifai Methods for Measurement of LDL-Cholesterol: A Critical Assessment of Direct Measurement by Homogeneous Assays versus Calculation Clin. Chem., February 1, 2002; 48(2): 236 - 254. [Abstract] [Full Text] [PDF]
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 N. Matsuoka, Y. Ogawa, H. Masuzaki, K. Ebihara, M. Aizawa-Abe, N. Satoh, E. Ishikawa, Y. Fujisawa, A. Kosaki, K. Yamada, et al. Decreased triglyceride-rich lipoproteins in transgenic skinny mice overexpressing leptin Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E334 - E339. [Abstract] [Full Text] [PDF]
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M. Esteban-Salan, A. Guimon-Bardesi, J. M. de la Viuda-Unzueta, M. N. Azcarate-Ania, P. Pascual-Usandizaga, and E. Amoroto-Del-Rio Analytical and Clinical Evaluation of Two Homogeneous Assays for LDL-Cholesterol in Hyperlipidemic Patients Clin. Chem., August 1, 2000; 46(8): 1121 - 1131. [Abstract] [Full Text] [PDF]
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