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Endocrinology and Metabolism |
1
Department of Pediatrics, Washington University School of Medicine, One Children's Place, St. Louis, MO 63110.
2
Linco Research, Inc., St. Charles, MO 63304.
3
Department of Nutrition, University of California-Davis,
Davis, CA 95616.
4
Divisions of Endocrinology and Research
Technology/Proteins, Lilly Research Laboratories, Eli Lilly and Co.,
Indianapolis, IN 46285.
a Author for correspondence. Fax 314-454-2274; e-mail landt{at}kids.wustl.edu.
Adipose tissue secretes leptin, which interacts with receptors in the hypothalamus. In rodent models of obesity, leptin increases metabolism and decreases food intake, which helps to maintain normal body composition. Accurate and precise methods to quantitate circulating leptin concentrations are needed for physiological studies. We developed an RIA to measure leptin in rat plasma, serum, or adipocyte culture fluids. The working range of the assay, defined by the detection limit and the highest calibrator, was 0.5–50 µg/L. Recovery of 1.6–11.6 µg/L leptin added to serum was 92–103%. The rat leptin RIA correlated well with a previously developed mouse RIA when rat plasma was assayed with both methods (r = 0.94), but the mouse leptin assay underestimated rat leptin in plasma. Within- and between-run CVs were 2.4% to 5.7%. Plasma leptin concentrations correlated directly with percentage of body fat, and correlation improved when the results were separated by gender (r = 0.796, P <0.001 for males; r = 0.710, P <0.001 for females). Leptin concentrations were generally higher in male rats than in females; plasma leptin increased 0.60 µg/L for each percentage of increase in body fat for males but only 0.22 µg/L for females. We conclude that rat serum/plasma leptin concentrations are accurately and precisely measured with this new RIA.
The following articles in journals at HighWire Press have cited this article:
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  M. H. Vickers, P. D. Gluckman, A. H. Coveny, P. L. Hofman, W. S. Cutfield, A. Gertler, B. H. Breier, and M. Harris The Effect of Neonatal Leptin Treatment on Postnatal Weight Gain in Male Rats Is Dependent on Maternal Nutritional Status during Pregnancy Endocrinology, April 1, 2008; 149(4): 1906 - 1913. [Abstract] [Full Text] [PDF]
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 M. Herrid, V. L. Nguyen, G. Hinch, and J. R McFarlane Leptin has concentration and stage-dependent effects on embryonic development in vitro. Reproduction, August 1, 2006; 132(2): 247 - 256. [Abstract] [Full Text] [PDF]
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A. M. Rodriguez, P. Roca, M. L. Bonet, C. Pico, P. Oliver, and A. Palou Positive correlation of skeletal muscle UCP3 mRNA levels with overweight in male, but not in female, rats Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2003; 285(4): R880 - R888. [Abstract] [Full Text] [PDF]
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 P. J. Havel Peripheral Signals Conveying Metabolic Information to the Brain: Short-Term and Long-Term Regulation of Food Intake and Energy Homeostasis Experimental Biology and Medicine, December 1, 2001; 226(11): 963 - 977. [Abstract] [Full Text] [PDF]
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J. E. McMinn, D. K. Sindelar, P. J. Havel, and M. W. Schwartz Leptin Deficiency Induced by Fasting Impairs the Satiety Response to Cholecystokinin Endocrinology, December 1, 2000; 141(12): 4442 - 4448. [Abstract] [Full Text] [PDF]
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J. E. McMinn, C. W. Wilkinson, P. J. Havel, S. C. Woods, and M. W. Schwartz Effect of intracerebroventricular alpha -MSH on food intake, adiposity, c-Fos induction, and neuropeptide expression Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2000; 279(2): R695 - R703. [Abstract] [Full Text] [PDF]
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J. T. Smith and B. J. Waddell Increased Fetal Glucocorticoid Exposure Delays Puberty Onset in Postnatal Life Endocrinology, July 1, 2000; 141(7): 2422 - 2428. [Abstract] [Full Text] [PDF]
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 D. A Ainslie, J. Proietto, B. C Fam, and A. W Thorburn Short-term, high-fat diets lower circulating leptin concentrations in rats1 Am. J. Clinical Nutrition, February 1, 2000; 71(2): 438 - 442. [Abstract] [Full Text] [PDF]
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P. J. Havel, J. Y. Uriu-Hare, T. Liu, K. L. Stanhope, J. S. Stern, C. L. Keen, and B. Ahren Marked and rapid decreases of circulating leptin in streptozotocin diabetic rats: reversal by insulin Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1482 - R1491. [Abstract] [Full Text] [PDF]
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