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Automation and Analytical Techniques |
1
Laboratoire d'Immunologie, Centre Hospitalier Lyon Sud, 69495 Pierre-Bénite, France.
2
Laboratorio di Chimica Clinica ed Ematologia, Ospedale
Civile Maggiore 37126 Verona, Italy.
3
Beckman Instruments Inc., 200 S. Kraemer Blvd., Brea, CA
92621.
4
Laboratorio Analisi, Ospedale di Stradella, 27049
Stradella, Italy.
5
Beckman Analytical SPA, Via Roma, 108, 20060 Cassina De
Pecchi, Italy.
a Author for correspondence. Fax +39 385 582 285; e-mail f.aguzzi{at}iol.it.
Serum protein electrophoresis and typing of monoclonal components (MCs)
are routine but time-consuming and technically demanding assays. We
evaluated capillary electrophoresis (Paragon CZETM 2000)
for automation of the two assays. CZE and cellulose acetate
electrophoresis gave similar data on 794 samples. Within-run and
between-run CVs were <2% for albumin and 
-globulins and 4–7% for
1-, 2-, and ß-globulins. Bilirubin,
hemoglobin, triglycerides, and fibrinogen were found not to interfere.
No carryover by capillaries was detected. The detection limit for MC
was <0.5 g/L. MC assessment by immunosubtraction on 403 samples
identified the monoclonal type in all samples with peak concentrations
>10 g/L; only 50% of MCs that could not be quantified by
densitometric scan were typed.
The following articles in journals at HighWire Press have cited this article:
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  P. Luraschi, I. Infusino, I. Zorzoli, G. Merlini, C. Fundaro and, and C. Franzini Heavy Chain Disease Can Be Detected by Capillary Zone Electrophoresis Clin. Chem., January 1, 2005; 51(1): 247 - 249. [Full Text] [PDF]
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H. Zetterberg and H. Nilsson-Ehle False-Negative Result in the Detection of an IgM Monoclonal Protein by Capillary Zone Electrophoresis Clin. Chem., October 1, 2004; 50(10): 1878 - 1880. [Full Text] [PDF]
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C. Gay-Bellile, D. Bengoufa, P. Houze, D. Le Carrer, M. Benlakehal, B. Bousquet, B. Gourmel, and T. Le Bricon Automated Multicapillary Electrophoresis for Analysis of Human Serum Proteins Clin. Chem., November 1, 2003; 49(11): 1909 - 1915. [Abstract] [Full Text] [PDF]
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 E B Duly, S Grimason, P Grimason, G Barnes, and T R Trinick Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods J. Clin. Pathol., October 1, 2003; 56(10): 780 - 781. [Abstract] [Full Text] [PDF]
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M. Jonsson and J. Carlson Computer-supported Interpretation of Protein Profiles after Capillary Electrophoresis Clin. Chem., July 1, 2002; 48(7): 1084 - 1093. [Abstract] [Full Text] [PDF]
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X. Bossuyt and W. E. Peetermans Effect of Piperacillin-Tazobactam on Clinical Capillary Zone Electrophoresis of Serum Proteins Clin. Chem., January 1, 2002; 48(1): 204 - 205. [Full Text] [PDF]
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X. Bossuyt and G. Marien False-Negative Results in Detection of Monoclonal Proteins by Capillary Zone Electrophoresis: A Prospective Study Clin. Chem., August 1, 2001; 47(8): 1477 - 1479. [Full Text] [PDF]
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R. Claeys, C. Groven, and F. K. Gorus Capillary Zone Electrophoresis of Proteins in Body Fluids: Comparison of Capillary and Agarose Gel Electrophoresis Clin. Chem., May 1, 2001; 47(5): 967 - 970. [Full Text] [PDF]
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K. Matsuda, N. Hiratsuka, T. Koyama, Y. Kurihara, O. Hotta, Y. Itoh, and K. Shiba Sensitive Method for Detection and Semiquantification of Bence Jones Protein by Cellulose Acetate Membrane Electrophoresis Using Colloidal Silver Staining Clin. Chem., April 1, 2001; 47(4): 763 - 766. [Full Text] [PDF]
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M. Jonsson, J. Carlson, J.-O. Jeppsson, and P. Simonsson Computer-supported Detection of M-Components and Evaluation of Immunoglobulins after Capillary Electrophoresis Clin. Chem., January 1, 2001; 47(1): 110 - 117. [Abstract] [Full Text] [PDF]
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M. L. Arranz-Pena, M. Gonzalez-Sagrado, A. M. Olmos-Linares, N. Fernandez-Garcia, and F. J. Martin-Gil Interference of Iodinated Contrast Media in Serum Capillary Zone Electrophoresis Clin. Chem., May 1, 2000; 46(5): 736 - 737. [Full Text] [PDF]
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X. Bossuyt, A. Mewis, and N. Blanckaert Interference of Radio-opaque Agents in Clinical Capillary Zone Electrophoresis Clin. Chem., January 1, 1999; 45(1): 129 - 131. [Full Text] [PDF]
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D. F. Keren Detection and Characterization of Monoclonal Componentsin Serum and Urine Clin. Chem., June 1, 1998; 44(6): 1143 - 1145. [Full Text] [PDF]
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