| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Molecular Pathology and Genetics |
1
Thalassemia Research Center, Institute of Science and Technology for Research and Development, Division of Hematology, Department of Medicine, Mahidol University, Nakornpathom 73170, Thailand.
2
Department of Obstetrics and Gynecology, Faculty of
Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700,
Thailand.
3
Department of Pathology, Faculty of Medicine Ramathibodi
Hospital, Mahidol University, Bangkok 10400, Thailand.
4
Bio-Rad Laboratories, 9810 Nazarette, Belgium.
a Address correspondence to this author at: Thalassemia Research Center, Institute of Science and Technology for Research and Development, Mahidol University, Salaya Campus, Puttamonthon 4 Rd., Nakornpathom 73170, Thailand. Fax 662-889-2559; e-mail grsfc{at}mahidol.ac.th.
The conventional approach to qualitative and quantitative analyses of
hemoglobin (Hb) molecules for the diagnoses of hemoglobinopathies
requires a combination of tests. We used an automated HPLC
(VARIANT(TM)) system to study 
-thalassemia and
ß-thalassemia syndromes in Thailand. The beta-thalassemia short
program is applicable to the diagnosis of -thalassemia and
ß-thalassemia disorders, including Hb H, EA Bart's disease, and EF
Bart's disease, in adults, newborns, and fetuses. The system cannot
quantify accurately certain Hb molecules, such as Hb H and Hb Bart's.
The alpha-thalassemia short program was therefore developed and used to
quantify Hb Bart's to detect -thalassemia genotypes in cord blood.
This automated HPLC system is an alternative approach to the diagnosis
of complicated thalassemia syndromes in Thailand and Southeast Asia.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  D. D. Mais, R. D. Gulbranson, and D. F. Keren The Range of Hemoglobin A2 in Hemoglobin E Heterozygotes as Determined by Capillary Electrophoresis Am J Clin Pathol, July 1, 2009; 132(1): 34 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Mosca, R Paleari, G Ivaldi, R Galanello, and P C Giordano The role of haemoglobin A2 testing in the diagnosis of thalassaemias and related haemoglobinopathies J. Clin. Pathol., January 1, 2009; 62(1): 13 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Banerjee, S. B. Chakrabarty, S. R. Karmakar, A. Chakrabarty, S. J. Biswas, S. Haque, D. Das, S. Paul, B. Mandal, B. Naoual, et al. Can Homeopathy Bring Additional Benefits to Thalassemic Patients on Hydroxyurea Therapy? Encouraging Results of a Preliminary Study Evid. Based Complement. Altern. Med., October 29, 2007; (2007) nem161v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Winichagoon, S. Svasti, P. Winichagoon, C. Chitchumroonchokchai, and S. Fucharoen Expression of {beta}E and {gamma}-globin genes in infants heterozygous for hemoglobin E and double heterozygous for hemoglobin E and {alpha}-thalassemia Haematologica, May 1, 2007; 92(5): 702 - 703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Joutovsky, J. Hadzi-Nesic, and M. A. Nardi HPLC Retention Time as a Diagnostic Tool for Hemoglobin Variants and Hemoglobinopathies: A Study of 60000 Samples in a Clinical Diagnostic Laboratory Clin. Chem., October 1, 2004; 50(10): 1736 - 1747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X M Xu, Y Q Zhou, G X Luo, C Liao, M Zhou, P Y Chen, J P Lu, S Q Jia, G F Xiao, X Shen, et al. The prevalence and spectrum of {alpha} and {beta} thalassaemia in Guangdong Province: implications for the future health burden and population screening J. Clin. Pathol., May 1, 2004; 57(5): 517 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Ou-Yang, L Hua, Q H Mo, and X M Xu Rapid, accurate genotyping of the common -{alpha}4.2 thalassaemia deletion based on the use of denaturing HPLC J. Clin. Pathol., February 1, 2004; 57(2): 159 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-Z. Liu, K. S. Tsang, C. K. Li, R. A. Shaw, and H. H. Mantsch Infrared Spectroscopic Identification of {beta}-Thalassemia Clin. Chem., July 1, 2003; 49(7): 1125 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Chotivanich, R. Udomsangpetch, K. Pattanapanyasat, W. Chierakul, J. Simpson, S. Looareesuwan, and N. White Hemoglobin E: a balanced polymorphism protective against high parasitemias and thus severe P falciparum malaria Blood, July 30, 2002; 100(4): 1172 - 1176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Clarke and T. N. Higgins Laboratory Investigation of Hemoglobinopathies and Thalassemias: Review and Update Clin. Chem., August 1, 2000; 46(8): 1284 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. P.L. van der Dijs, M. Volmer, D. G. van Gijssel-Wiersma, J. W. Smit, R. van Veen, and F. A.J. Muskiet Predictive Value of Cord Blood Hematological Indices and Hemoglobin Barts for the Detection of Heterozygous {alpha}-Thalassemia-2 in an African-Caribbean Population Clin. Chem., September 1, 1999; 45(9): 1495 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Campbell, J. S. Henthorn, and S. C. Davies Evaluation of Cation-Exchange HPLC Compared with Isoelectric Focusing for Neonatal Hemoglobinopathy Screening Clin. Chem., July 1, 1999; 45(7): 969 - 975. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
