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Enzymes and Protein Markers |
a Address correspondence to this author at: Central Clinical Laboratory, Clinical Pathology Department, University Hospital Leuven, Kapucijnenvoer 33, B-3000 Leuven, Belgium. Fax 32 16 332896; e-mail xavier.bossuyt{at}uz.kuleuven.ac.be.
We compared the automated Paragon 2000 clinical capillary zone
electrophoresis (CZE) system with two manual methods, agarose
electrophoresis (AGE) and cellulose acetate electrophoresis (CAE).
Reference intervals in healthy adults were determined for each method.
When compared with AGE and CAE, CZE gave substantially higher reference
values for the 
1-globulin fraction. With CZE, within-run
precision for fraction quantitation was between 0.5% (albumin) and
4.1% (1-globulin). Total precision was between 0.8%
(albumin) and 5.3% (ß-globulin). Data obtained from CZE showed poor
linear correlation with results obtained by AGE but good linear
correlation with data from CAE. Analysis of serum from patients with
inter alia inflammation, nephrotic syndrome, or polyclonal gammopathy
showed that clinical information obtained by CZE is comparable with
information obtained by AGE and CAE. We conclude that CZE offers a
clinically reliable alternative to AGE and CAE and has the advantages
of automation, higher precision, and faster turnaround time.
The following articles in journals at HighWire Press have cited this article:
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  Y. Roman, M.-C. Bomsel-Demontoy, J. Levrier, D. Chaste-Duvernoy, and M. S. Jalme EFFECT OF HEMOLYSIS ON PLASMA PROTEIN LEVELS AND PLASMA ELECTROPHORESIS IN BIRDS J. Wildl. Dis., January 1, 2009; 45(1): 73 - 80. [Abstract] [Full Text] [PDF]
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 E. Van Hoeyveld, L. Dupont, and X. Bossuyt Quantification of IgG Antibodies to Aspergillus fumigatus and Pigeon Antigens by ImmunoCAP Technology: An Alternative to the Precipitation Technique? Clin. Chem., September 1, 2006; 52(9): 1785 - 1793. [Abstract] [Full Text] [PDF]
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G. van der Watt and P. Berman Pseudoparaproteinemia after Iopamidol Infusion for Coronary Angiography Clin. Chem., January 1, 2005; 51(1): 273 - 274. [Full Text] [PDF]
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B. Van Meensel, M. Hiele, I. Hoffman, S. Vermeire, P. Rutgeerts, K. Geboes, and X. Bossuyt Diagnostic Accuracy of Ten Second-Generation (Human) Tissue Transglutaminase Antibody Assays in Celiac Disease Clin. Chem., November 1, 2004; 50(11): 2125 - 2135. [Abstract] [Full Text] [PDF]
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C. Gay-Bellile, D. Bengoufa, P. Houze, D. Le Carrer, M. Benlakehal, B. Bousquet, B. Gourmel, and T. Le Bricon Automated Multicapillary Electrophoresis for Analysis of Human Serum Proteins Clin. Chem., November 1, 2003; 49(11): 1909 - 1915. [Abstract] [Full Text] [PDF]
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X. Bossuyt, J. Verhaegen, G. Marien, and N. Blanckaert Effect of Sulfamethoxazole on Clinical Capillary Zone Electrophoresis of Serum Proteins Clin. Chem., February 1, 2003; 49(2): 340 - 341. [Full Text] [PDF]
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M. Jonsson and J. Carlson Computer-supported Interpretation of Protein Profiles after Capillary Electrophoresis Clin. Chem., July 1, 2002; 48(7): 1084 - 1093. [Abstract] [Full Text] [PDF]
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X. Bossuyt and W. E. Peetermans Effect of Piperacillin-Tazobactam on Clinical Capillary Zone Electrophoresis of Serum Proteins Clin. Chem., January 1, 2002; 48(1): 204 - 205. [Full Text] [PDF]
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X. Bossuyt and G. Marien False-Negative Results in Detection of Monoclonal Proteins by Capillary Zone Electrophoresis: A Prospective Study Clin. Chem., August 1, 2001; 47(8): 1477 - 1479. [Full Text] [PDF]
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R. Claeys, C. Groven, and F. K. Gorus Capillary Zone Electrophoresis of Proteins in Body Fluids: Comparison of Capillary and Agarose Gel Electrophoresis Clin. Chem., May 1, 2001; 47(5): 967 - 970. [Full Text] [PDF]
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M. Jonsson, J. Carlson, J.-O. Jeppsson, and P. Simonsson Computer-supported Detection of M-Components and Evaluation of Immunoglobulins after Capillary Electrophoresis Clin. Chem., January 1, 2001; 47(1): 110 - 117. [Abstract] [Full Text] [PDF]
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M. L. Arranz-Pena, M. Gonzalez-Sagrado, A. M. Olmos-Linares, N. Fernandez-Garcia, and F. J. Martin-Gil Interference of Iodinated Contrast Media in Serum Capillary Zone Electrophoresis Clin. Chem., May 1, 2000; 46(5): 736 - 737. [Full Text] [PDF]
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M.-L. Chen and C.-W. Lam Protein Zone Electrophoresis of Pleural Effusion: The Diagnostic Separation of Transudates and Exudates Clin. Chem., October 1, 1999; 45(10): 1882 - 1885. [Full Text] [PDF]
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X. Bossuyt, A. Mewis, and N. Blanckaert Interference of Radio-opaque Agents in Clinical Capillary Zone Electrophoresis Clin. Chem., January 1, 1999; 45(1): 129 - 131. [Full Text] [PDF]
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X. Bossuyt, A. Bogaerts, G. Schiettekatte, and N. Blanckaert Detection and classification of paraproteins by capillary immunofixation/subtraction Clin. Chem., April 1, 1998; 44(4): 760 - 764. [Abstract] [Full Text] [PDF]
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