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Clinical Chemistry 44: 773-778, 1998;
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Right arrow Molecular Diagnostics and Genetics
Right arrow Lipids, Lipoproteins, and Cardiovascular Risk Factors
(Clinical Chemistry. 1998;44:773-778.)
© 1998 American Association for Clinical Chemistry, Inc.

Lipids and Lipoproteins

A competitive reverse transcription–PCR to study apolipoprotein {epsilon} gene expression

Martin Rexin, and Giso Feussnera

Department of Internal Medicine I, University of Heidelberg, Endocrinology and Metabolism, Bergheimer Strasse 58, D-69115 Heidelberg, Germany.
a Address correspondence to this author at: Medizinische Universitätsklinik Heidelberg, Abteilung Innere Medizin I, Endokrinologie und Stoffwechsel, Bergheimer Strasse 58, D-69115 Heidelberg. Fax 49 6221 56 53 42; e-mail uk691jd{at}genius.embnet.dkfz-heidelberg.de.

We developed a rapid and simple competitive reverse transcription-polymerase chain reaction for the quantification of apo{epsilon} mRNA in human monocyte-derived macrophages. The method was applied, and its reliability was shown in patients with the familial lipoprotein disorder, type III hyperlipoproteinemia. Type III hyperlipoproteinemic patients express markedly higher concentrations of apo{epsilon} mRNA when compared with healthy controls. Patients with this disease are usually (>90%) homozygous for a receptor binding-defective isoform of apolipoprotein apo E (apo E2). The higher expression of apo{epsilon} mRNA in the patients could, therefore, be a physiological mechanism to compensate for functionally defective apo E. The developed procedure might be valuable in assessment of apo{epsilon} gene expression in human disease.




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Copyright © 1998 by the American Association for Clinical Chemistry.