Clinical Chemistry
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Clinical Chemistry 44: 1191-1197, 1998;
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(Clinical Chemistry. 1998;44:1191-1197.)
© 1998 American Association for Clinical Chemistry, Inc.

Enzymes and Protein Markers

Sodium dodecyl sulfate-agarose gel electrophoresis of urinary proteins: application to multiple myeloma

Thierry Le Bricon1,a, Danielle Erlich1, Djaouida Bengoufa2, Michelle Dussaucy1, Jean-Pierre Garnier1, and Bernard Bousquet1

1 Laboratoires de Biochimie A et B and
2 Laboratoire d'Immunologie, Hôpital St-Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France.
a Author for correspondence. Fax 33 1 42 49 92 47; e-mail Biochimie-a{at}chu-stlouis.fr.

We evaluated a new sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) for urinary protein analysis in patients with multiple myeloma (MM; n = 47; ages, 62 ± 2 years, mean ± SE). Abnormal proteinuria (mean = 1872 ± 360 mg/24 h) was present in 95% of the samples; 75% of the patients had some sign of renal dysfunction (glomerular and/or tubular) according to their SDS-AGE pattern. A band suggesting Bence Jones proteinuria (BJP) was detected in 40 vs 33 specimens by routine AGE. Immunofixation identified BJP in 38 patients; the calculated sensitivity of SDS-AGE for BJP was 97%. Excellent correlation (P <0.0001) was obtained with routine AGE (r = 0.994) and immunonephelometry (r = 0.963) for light chain quantification. SDS-AGE allows easy evaluation of renal dysfunction and shows high sensitivity for BJP detection. In a specialized laboratory, it is useful for following the progress of MM patients through the semiquantification of BJP.




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