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Enzymes and Protein Markers |
1
EP Central Hospital Laboratory, FIN-60220 Seinäjoki, Finland, and
2
Departments of Clinical Chemistry, University of Helsinki and Oulu, Fin-90220 Oulu, Finland.
a Author for correspondence. Fax 358-6-415-4924.
Carbohydrate-deficient transferrin (CDT) has been suggested as a
specific marker of alcohol abuse. We designed this study to compare the
conventional CDTect method (Pharmacia & Upjohn) and the new
semiautomated Axis %CDT turbidimetric immunoassay (%CDT TIA) for
their diagnostic performance to identify problem drinking. The
sensitivities of the %CDT TIA and CDTect for correctly classifying
heavy drinkers (n = 90) were 29% and 59% with the thresholds
currently recommended by the manufacturers, respectively. In the
control group (n = 114), which included hospitalized patients with
abnormal serum transferrin concentrations, the CDTect assay gave 21
false-positive values (18%), whereas the %CDT TIA showed 100%
specificity. With the cutoff limits based on the present healthy
control group (mean + 2 SD), the sensitivities of the %CDT TIA and
CDTect were 61% and 86%, respectively. For men, the ROC plot area of
the CDTect results in comparisons of alcohol abusers and healthy
controls was significantly (P <0.05) higher than that of
the %CDT TIA results, whereas for women, there was no significant
difference in this respect. The slope and intercept (with 95%
confidence intervals) for linear regression between CDTect and %CDT
TIA were 0.13 (0.12–0.15) and 1.16 (0.73–1.59), respectively
(Sy
x = 1.51, r = 0.744). CDTect
results correlated positively with serum transferrin
(r = 0.224, P <0.001), whereas the %CDT
TIA results showed a slight inverse correlation with serum transferrin
(r = -0.132, P = 0.07). The data
suggest that CDTect is more sensitive than %CDT TIA in detecting
drinking problems. However, the %CDT TIA method yields more
specificity when analyzing samples from patients with high serum
transferrin concentrations.
The following articles in journals at HighWire Press have cited this article:
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  J. B. Whitfield, V. Dy, P. A.F. Madden, A. C. Heath, N. G. Martin, and G. W. Montgomery Measuring Carbohydrate-Deficient Transferrin by Direct Immunoassay: Factors Affecting Diagnostic Sensitivity for Excessive Alcohol Intake Clin. Chem., July 1, 2008; 54(7): 1158 - 1165. [Abstract] [Full Text] [PDF]
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 J. HIETALA, H. KOIVISTO, P. ANTTILA, and O. NIEMELA COMPARISON OF THE COMBINED MARKER GGT-CDT AND THE CONVENTIONAL LABORATORY MARKERS OF ALCOHOL ABUSE IN HEAVY DRINKERS, MODERATE DRINKERS AND ABSTAINERS Alcohol Alcohol., September 1, 2006; 41(5): 528 - 533. [Abstract] [Full Text] [PDF]
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P. Anttila, K. Jarvi, J. Latvala, and O. Niemela METHOD-DEPENDENT CHARACTERISTICS OF CARBOHYDRATE-DEFICIENT TRANSFERRIN MEASUREMENTS IN THE FOLLOW-UP OF ALCOHOLICS Alcohol Alcohol., January 1, 2004; 39(1): 59 - 63. [Abstract] [Full Text] [PDF]
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P. Anttila, K. Jarvi, J. Latvala, J. E. Blake, and O. Niemela DIAGNOSTIC CHARACTERISTICS OF DIFFERENT CARBOHYDRATE-DEFICIENT TRANSFERRIN METHODS IN THE DETECTION OF PROBLEM DRINKING: EFFECTS OF LIVER DISEASE AND ALCOHOL CONSUMPTION Alcohol Alcohol., September 1, 2003; 38(5): 415 - 420. [Abstract] [Full Text] [PDF]
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F. J. Legros, V. Nuyens, M. Baudoux, K. Zouaoui Boudjeltia, J.-L. Ruelle, J. Colicis, F. Cantraine, and J.-P. Henry Use of Capillary Zone Electrophoresis for Differentiating Excessive from Moderate Alcohol Consumption Clin. Chem., March 1, 2003; 49(3): 440 - 449. [Abstract] [Full Text] [PDF]
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F. J. Legros, V. Nuyens, E. Minet, P. Emonts, K. Z. Boudjeltia, A. Courbe, J.-L. Ruelle, J. Colicis, F. de L'Escaille, and J.-P. Henry Carbohydrate-deficient Transferrin Isoforms Measured by Capillary Zone Electrophoresis for Detection of Alcohol Abuse Clin. Chem., December 1, 2002; 48(12): 2177 - 2186. [Abstract] [Full Text] [PDF]
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F. Tagliaro, F. Bortolotti, R. M. Dorizzi, M. Marigo, J. R. Delanghe, B. Wuyts, and M. L. De Buyzere Caveats in Carbohydrate-deficient Transferrin Determination Drs. Delanghe, Wuyts, and De Buyzere respond: Clin. Chem., January 1, 2002; 48(1): 208 - 209. [Full Text] [PDF]
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R. F. Anton, C. Dominick, M. Bigelow, and C. Westby Comparison of Bio-Rad %CDT TIA and CDTect as Laboratory Markers of Heavy Alcohol Use and Their Relationships with {gamma}-Glutamyltransferase Clin. Chem., October 1, 2001; 47(10): 1769 - 1775. [Abstract] [Full Text] [PDF]
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A. Helander, M. Fors, and B. Zakrisson STUDY OF AXIS-SHIELD NEW %CDT IMMUNOASSAY FOR QUANTIFICATION OF CARBOHYDRATE-DEFICIENT TRANSFERRIN (CDT) IN SERUM Alcohol Alcohol., September 1, 2001; 36(5): 406 - 412. [Abstract] [Full Text] [PDF]
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A. Berkowicz, S. Wallerstedt, K. Wall, and H. Denison Carbohydrate-deficient transferrin in vitreous humour: a marker of possible withdrawal-related death in alcoholics Alcohol Alcohol., May 1, 2001; 36(3): 231 - 234. [Abstract] [Full Text] [PDF]
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T. Arndt Carbohydrate-deficient Transferrin as a Marker of Chronic Alcohol Abuse: A Critical Review of Preanalysis, Analysis, and Interpretation Clin. Chem., January 1, 2001; 47(1): 13 - 27. [Abstract] [Full Text] [PDF]
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A. Helander Absolute or Relative Measurement of Carbohydrate-deficient Transferrin in Serum? Experiences with Three Immunological Assays Clin. Chem., January 1, 1999; 45(1): 131 - 135. [Full Text] [PDF]
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