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Enzymes and Protein Markers |
Departments of
1
Medical Chemistry and Biochemistry,
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Cardiac Surgery, and
3
Anaethesia and General Intensive Care Medicine, University of Innsbruck School of Medicine, A-6020 Innsbruck, Austria.
a Address correspondence to this author at: Institut für Medizinische Chemie und Biochemie, Fritz-Preglstrasse 3, A-6020 Innsbruck, Austria. Fax 43-512-507-2876; e-mail Johannes.Mair{at}uibk.ac.at.
We investigated the net myocardial release of creatine kinase isoenzyme MB (CKMB), myoglobin, cardiac troponin T (cTnT), cardiac troponin I (cTnI), and cardiac ß-type myosin heavy chain (ß-MHC) into the coronary circulation after cardioplegic cardiac arrest in humans. Cardiac markers were measured in paired arterial, central venous, and coronary sinus blood in 19 patients undergoing elective coronary artery bypass grafting (CABG) before aortic cross-clamping and 1, 5, 10, and 20 min after aortic declamping. cTnT and cTnI were released into the coronary sinus in parallel to each other and almost simultaneously to myoglobin and CKMB within 20 min of reperfusion. In contrast, no ß-MHC was released in the same patients during the study period. The average soluble cTnT and cTnI pools in right atrial appendages of 11 patients with right atrial and right ventricular pressures within reference values were comparable and were ~8% of total myocardial troponin content. The soluble ß-MHC pool was <0.1% in all patients. Our results demonstrate the impact of the different intracellular compartmention of regulatory and contractile proteins on their early release from damaged myocardium.
Key Words: cTnT, cardiac troponin T • cTnI, cardiac troponin I • CK, creatine kinase • CKMB, creatine kinase MB isoenzyme • MHC, myosin heavy chain • ß-MHC, cardiac ß-type heavy chain myosin • LD, lactate dehydrogenase • CABG, coronary artery by-pass grafting.
The following articles in journals at HighWire Press have cited this article:
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  D. Abramov, M. Abu-Tailakh, M. Frieger, A. Ganiel, D. Tuvbin, and A. Wolak Plasma Troponin Levels After Cardiac Surgery vs After Myocardial Infarction Asian Cardiovasc Thorac Ann, December 1, 2006; 14(6): 530 - 535. [Abstract] [Full Text] [PDF]
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 M. Codispoti, T. Sundaramoorthi, R. A. Saad, A. Reid, C. Sinclair, and P. Mankad Optimal myocardial protection strategy for coronary artery bypass grafting without cardioplegia: prospective randomised trial Interactive CardioVascular and Thoracic Surgery, June 1, 2006; 5(3): 217 - 221. [Abstract] [Full Text] [PDF]
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 M. Kuduvalli, N. Newall, A. Stott, A. D. Grayson, and B. M. Fabri Impact of avoiding cardiopulmonary bypass for coronary surgery on perioperative cardiac enzyme release and survival. Eur. J. Cardiothorac. Surg., May 1, 2006; 29(5): 729 - 735. [Abstract] [Full Text] [PDF]
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A. Gurbuz, B. Emrecan, L. Yilik, I. Ozsoyler, M. Kestelli, C. Ozbek, and N. Karahan Intracoronary shunt reduces postoperative troponin leaks: a prospective randomized study Eur. J. Cardiothorac. Surg., February 1, 2006; 29(2): 186 - 189. [Abstract] [Full Text] [PDF]
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 V. Kedar, H. McDonough, R. Arya, H.-H. Li, H. A. Rockman, and C. Patterson Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I PNAS, December 28, 2004; 101(52): 18135 - 18140. [Abstract] [Full Text] [PDF]
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 J. A. Simpson, J. Van Eyk, and S. Iscoe Respiratory muscle injury, fatigue and serum skeletal troponin I in rat J. Physiol., February 1, 2004; 554(3): 891 - 903. [Abstract] [Full Text] [PDF]
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 J. B. Selvanayagam, S. E. Petersen, J. M. Francis, M. D. Robson, A. Kardos, S. Neubauer, and D. P. Taggart Effects of Off-Pump Versus On-Pump Coronary Surgery on Reversible and Irreversible Myocardial Injury: A Randomized Trial Using Cardiovascular Magnetic Resonance Imaging and Biochemical Markers Circulation, January 27, 2004; 109(3): 345 - 350. [Abstract] [Full Text] [PDF]
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 K. B. Wallace, E. Hausner, E. Herman, G. D. Holt, J. T. Macgregor, A. L. Metz, E. Murphy, I.Y. Rosenblum, F. D. Sistare, and M. J. York Serum Troponins as Biomarkers of Drug-Induced Cardiac Toxicity Toxicol Pathol, January 1, 2004; 32(1): 106 - 121. [PDF]
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 R. Labugger, J. A. Simpson, M. Quick, H. A. Brown, C. E. Collier, I. Neverova, and J. E. Van Eyk Strategy for Analysis of Cardiac Troponins in Biological Samples with a Combination of Affinity Chromatography and Mass Spectrometry Clin. Chem., June 1, 2003; 49(6): 873 - 879. [Abstract] [Full Text] [PDF]
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 N. Nesher, E. Zisman, T. Wolf, R. Sharony, G. Bolotin, M. David, G. Uretzky, and R. Pizov Strict Thermoregulation Attenuates Myocardial Injury During Coronary Artery Bypass Graft Surgery as Reflected by Reduced Levels of Cardiac-Specific Troponin I Anesth. Analg., February 1, 2003; 96(2): 328 - 335. [Abstract] [Full Text] [PDF]
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 B. J. Freda, W. H. W. Tang, F. Van Lente, W. F. Peacock, and G. S. Francis Cardiac troponins in renal insufficiency: Review and clinical implications J. Am. Coll. Cardiol., December 18, 2002; 40(12): 2065 - 2071. [Abstract] [Full Text] [PDF]
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 T. M. Welsh, G. D. Kukes, and L. M. Sandweiss Differences of Creatine Kinase MB and Cardiac Troponin I Concentrations in Normal and Diseased Human Myocardium Ann. Clin. Lab. Sci., January 1, 2002; 32(1): 44 - 49. [Abstract] [Full Text] [PDF]
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