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Clinical Chemistry 44: 1937-1941, 1998;
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(Clinical Chemistry. 1998;44:1937-1941.)
© 1998 American Association for Clinical Chemistry, Inc.

Drug Monitoring and Toxicology

Simple method for the routine determination of betaine and N,N-dimethylglycine in blood and urine

Maurice D. Laryeaa, Folkert Steinhagen, Sandra Pawliczek, and Udo Wendel

a Author for correspondence. Fax 049-0211-811910; e-mail Laryea{at}uni-duesseldorf.de.

A simple and convenient method using commercially available derivatization reagents is described for the measurement of betaine and N,N-dimethylglycine (DMG) in blood and urine. Precolumn derivatization of plasma or urine is performed directly in acetonitrile without extraction with p-bromophenacyl bromide and crown ether as catalyst. The p-bromophenacyl ester derivatives are then separated by high-performance liquid chromatography, using an isocratic system of acetonitrile and water containing choline. Effluent was monitored at 254 nm. The limit of detection was 5 µmol/L for betaine and 2 µmol/L for DMG. Analytical recovery was >97% for both analytes. Total and within-day CVs were 2.0–4.4% and 0.9–2.2% for DMG. For betaine, the total and within-day CVs were 1.3–5.3% and 0.4–3.8%, respectively. The method is precise and cost-effective and has been used successfully to determine the concentrations of DMG and betaine in human plasma and urine.




The following articles in journals at HighWire Press have cited this article:


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 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. Schafer, L. Hoffmann, K. Heldt, M. R. Lornejad-Schafer, G. Brauers, T. Gehrmann, T. A. Garrow, D. Haussinger, E. Mayatepek, B. C. Schwahn, et al.
Osmotic regulation of betaine homocysteine-S-methyltransferase expression in H4IIE rat hepatoma cells
Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G1089 - G1098.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
P. I. Holm, P. M. Ueland, G. Kvalheim, and E. A. Lien
Determination of Choline, Betaine, and Dimethylglycine in Plasma by a High-Throughput Method Based on Normal-Phase Chromatography-Tandem Mass Spectrometry
Clin. Chem., February 1, 2003; 49(2): 286 - 294.
[Abstract] [Full Text] [PDF]


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