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Clinical Chemistry 44: 1974-1979, 1998;
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(Clinical Chemistry. 1998;44:1974-1979.)
© 1998 American Association for Clinical Chemistry, Inc.

Clinical Immunology

Measurement of specific immunoglobulin E: intermethod comparison and standardization

Mario Plebania, Daniela Bernardi, Daniela Basso, Franco Borghesan, and Diego Faggian

Department of Laboratory Medicine, Azienda Ospedaliera di Padova, 35128 Padua, Italy.
a Address correspondence to this author at: Servizio di Medicina di Laboratorio, Azienda Ospedaliera di Padova, Via Giustiniani, 2, 35128 Padua, Italy. Fax 39-49-663240; e-mail mariopl{at}ux1.unipd.it.

Recently introduced "second-generation" techniques for specific IgE measurement have produced some analytical improvement, offering better clinical sensitivity than previous techniques. The aims of our study were to compare the analytical and clinical performances of four second-generation techniques for allergen-specific IgE measurement in serum and to ascertain whether the new system for reporting quantitative results contributes to greater clinical agreement between findings using the techniques considered. Allergen-specific IgE was measured using the CAP® System, CARLA®, ENEA®, and AlaSTAT®, and the findings were compared. A significant disagreement was found between CAP and ENEA for all allergens and between CAP and CARLA for D1 and G5. However, the clinical discrepancies were reduced by selecting method-specific thresholds using ROC analysis. Second-generation techniques enable us to obtain better standardization of results; however, the identification of a specific threshold appears to be a prerequisite for the appropriate clinical interpretation of the test findings.


Key Words: RAST, radio allergo sorbent test • kUa/L, kilounits of allergen-specific IgE per liter • AUC, area under the curve • CI, confidence interval.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
M. Ollert, S. Weissenbacher, J. Rakoski, and J. Ring
Allergen-Specific IgE Measured by a Continuous Random-Access Immunoanalyzer: Interassay Comparison and Agreement with Skin Testing
Clin. Chem., July 1, 2005; 51(7): 1241 - 1249.
[Abstract] [Full Text] [PDF]

Home page
 Annals of Clinical & Laboratory ScienceHome page
T. M. Li, T. Chuang, S. Tse, D. Hovanec-Burns, and A. S. El Shami
Development and Validation of a Third Generation Allergen-Specific IgE Assay on the Continuous Random Access IMMULITE(R) 2000 Analyzer
Ann. Clin. Lab. Sci., January 1, 2004; 34(1): 67 - 74.
[Abstract] [Full Text] [PDF]


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