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Clinical Chemistry 44: 2031-2035, 1998;
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(Clinical Chemistry. 1998;44:2031-2035.)
© 1998 American Association for Clinical Chemistry, Inc.

Oak Ridge Conference

Fixed polarizer ellipsometry for simple and sensitive detection of thin films generated by specific molecular interactions: applications in immunoassays and DNA sequence detection

Rachel M. Ostroff1,a, Diana Maul1, Gregory R. Bogart1, Shao Yang1, Jennifer Christian1, Deborah Hopkins1, David Clark1, Brian Trotter2, and Garret Moddel2

1 BioStar, Inc., Boulder, CO 80301.

2 Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309-0425.
a Address correspondence to this author at: BioStar, Inc., 6655 Lookout Rd., Boulder, CO 80301. Fax 303-530-6627; e-mail r_ostroff{at}biostar.com.


Abstract

Biological thin films may form on a surface by specific molecular interactions. The fixed polarizer ellipsometer (FPE) is a sensitive instrument that detects biological thin films either qualitatively or quantitatively. The design is simple and inexpensive. The assays are formatted on an optical surface, and the FPE detection is based on the phase shift of linearly polarized light after reflection through a thin film. We have constructed mathematical models of the FPE response to reflection through single-layer and two-layer films that agree closely with experimental data. Several biological assays have been measured with the FPE to demonstrate the application of this technology to clinical targets, including ultrasensitive immunoassays for hepatitis B surface antigen (0.1 ng/mL) and {alpha}-fetoprotein (0.01 ng/mL) and DNA hybridization (0.5 fmol/µL target probe). A clinical study for detection of group A streptococcus from patient throat swabs demonstrated the qualitative application of the FPE to infectious disease targets. The flexibility and sensitivity of the FPE makes this technology suitable for numerous target analytes and applications.


Key Words: FPE, fixed polarizer ellipsometer • TMB, tetramethylbenzidene • DLC, diamond-like carbon • HBsAg, hepatitis B surface antigen.




The following articles in journals at HighWire Press have cited this article:


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R. Jenison, H. La, A. Haeberli, R. Ostroff, and B. Polisky
Silicon-based Biosensors for Rapid Detection of Protein or Nucleic Acid Targets
Clin. Chem., October 1, 2001; 47(10): 1894 - 1900.
[Abstract] [Full Text] [PDF]

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R. Jenison, A. Haeberli, S. Yang, B. Polisky, and R. Ostroff
Thin Film Biosensor for Rapid Detection of mecA from Methicillin-resistant Staphylococcus aureus
Clin. Chem., September 1, 2000; 46(9): 1501 - 1504.
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R. M. Ostroff, D. Hopkins, A. B. Haeberli, W. Baouchi, and B. Polisky
Thin Film Biosensor for Rapid Visual Detection of Nucleic Acid Targets
Clin. Chem., September 1, 1999; 45(9): 1659 - 1664.
[Abstract] [Full Text] [PDF]


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