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Articles |
Departments of
1
Clinical Chemistry and
2
Urology, University Hospital Gent, De Pintelaan 185, B-9000 Gent, Belgium.
a Author for correspondence. Fax 32-9-2404985; e-mail joris.delanghe{at}rug.ac.be.
Recently, the Sysmex UF-100 flow cytometer was developed to automate urinalysis. We compared UF-100 test results with those of an automated dipstick reader. A cross-check of UF-100, dipstick, and microscopic sediment data was performed in 1001 urine samples. Good agreements (P <0.001) were obtained between UF-100 and dipstick data for erythrocytes (r = 0.636) and leukocytes (r = 0.785). Even in urine with low conductivity, the UF-100 could detect lysed erythrocytes. The UF-100 bacterial count was higher among nitrite-positive urine samples (P <0.0001) and was positively correlated with the UF-100 leukocyte count (r = 0.745; P <0.001). In stored urine (24 h), bacterial counts increased, whereas the forward light scatter of leukocytes decreased (P <0.01). Casts and yeast cells reported by the UF-100 should be confirmed by microscopic review because false positives occurred. We suggest that a computer-assisted cross-check of UF-100 and dipstick data allows a clinically acceptable sieving system to reduce the workload of microscopic sediment urinalysis.
The following articles in journals at HighWire Press have cited this article:
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  Y.-L. Chen, M.-K. Chang, Y.-J. Chen, and B.-C. Chang Comparing Neubauer Hemacytometer, SY Conventional, SY Located, and Automated Flow Cytometer F-100 Methods for Urinalysis Lab Med, April 1, 2009; 40(4): 227 - 231. [Abstract] [Full Text] [PDF]
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 M. Beullens, J. R. Delanghe, and M. Bollen False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise Blood, June 15, 2006; 107(12): 4711 - 4713. [Abstract] [Full Text] [PDF]
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 C. Ottiger and A. R. Huber Quantitative Urine Particle Analysis: Integrative Approach for the Optimal Combination of Automation with UF-100 and Microscopic Review with KOVA Cell Chamber Clin. Chem., April 1, 2003; 49(4): 617 - 623. [Abstract] [Full Text] [PDF]
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J. Penders, T. Fiers, and J. R. Delanghe Quantitative Evaluation of Urinalysis Test Strips Clin. Chem., December 1, 2002; 48(12): 2236 - 2241. [Abstract] [Full Text] [PDF]
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 J. R. Delanghe, M. R. Langlois, B. Wuyts, M. L. De Buyzere, Z. Zaman, J. Verhaegen, and S. Roggeman Performance of Urinary Flow Cytometry in Predicting Outcome of Urine Cultures J. Clin. Microbiol., June 1, 2002; 40(6): 2314 - 2315. [Full Text] [PDF]
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T. Kouri, L. Vuotari, S. Pohjavaara, and P. Laippala Preservation of Urine for Flow Cytometric and Visual Microscopic Testing Clin. Chem., June 1, 2002; 48(6): 900 - 905. [Abstract] [Full Text] [PDF]
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Z. Zaman, S. Roggeman, and J. Verhaegen Unsatisfactory Performance of Flow Cytometer UF-100 and Urine Strips in Predicting Outcome of Urine Cultures J. Clin. Microbiol., November 1, 2001; 39(11): 4169 - 4171. [Abstract] [Full Text] [PDF]
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 T. Apeland, O. Mestad, and O. Hetland Assessment of haematuria: automated urine flowmetry vs microscopy Nephrol. Dial. Transplant., August 1, 2001; 16(8): 1615 - 1619. [Abstract] [Full Text] [PDF]
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J. T. Van Acker, J. R. Delanghe, M. R. Langlois, Y. E. Taes, M. L. De Buyzere, and A. G. Verstraete Automated Flow Cytometric Analysis of Cerebrospinal Fluid Clin. Chem., March 1, 2001; 47(3): 556 - 560. [Abstract] [Full Text] [PDF]
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 A. Alvarez-Barrientos, J. Arroyo, R. Canton, C. Nombela, and M. Sanchez-Perez Applications of Flow Cytometry to Clinical Microbiology Clin. Microbiol. Rev., April 1, 2000; 13(2): 167 - 195. [Abstract] [Full Text] [PDF]
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