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Clinical Chemistry 45: 29-34, 1999;
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(Clinical Chemistry. 1999;45:29-34.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Identification of MEN1 Mutations in Sporadic Enteropancreatic Neuroendocrine Tumors by Analysis of Paraffin-embedded Tissue

Matthew D. Mailman1, Peter Muscarella2, William J. Schirmer2, E. Christopher Ellison2, Thomas M. O'Dorisio3 and Thomas W. Prior1,a

Departments of
1 Pathology,
2 Surgery, and
3 Internal Medicine, The Ohio State University College of Medicine, Columbus, OH 43210.
a Address correspondence to this author at: Department of Pathology, The Ohio State University, 121 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210. Fax 614-292-7072; e-mail prior-1{at}medctr.osu.edu.

Gastrinomas and other gastrointestinal neuroendocrine tumors may occur sporadically or as part of the inherited syndrome multiple endocrine neoplasia type 1 (MEN1). Mutations in the recently identified MEN1 gene have been described in sporadic gastrinomas and insulinomas. This study describes techniques used to identify mutations in the MEN1 gene in DNA extracted from paraffin-preserved tissue. Two novel mutations are identified in the MEN1 gene from nine archived paraffin-embedded neuroendocrine tumors, demonstrating that retrospective genetic analysis can be used to identify mutations in the MEN1 gene from preserved tissue. Conditions are provided by which paraffin-embedded tissue can be used as a source of genetic material for sequence information of sufficient quality for mutational studies of the MEN1 gene. It should also be possible to apply this retrospective genetic analysis of paraffin-embedded tissue to other disease models.




The following articles in journals at HighWire Press have cited this article:


Home page
 Mol. Endocrinol.Home page
S. S. Guo and M. P. Sawicki
Molecular and Genetic Mechanisms of Tumorigenesis in Multiple Endocrine Neoplasia Type-1
Mol. Endocrinol., October 1, 2001; 15(10): 1653 - 1664.
[Abstract] [Full Text] [PDF]


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